Related Experiment Video
Updated: Jan 23, 2026
11:00
Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
Published on: December 23, 2015
19.7K
T Cell: Primary Culture from Peripheral Blood
1Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Institute of the Ruhr-University, Bochum, Germany. raulf@ipa-dguv.de.
Methods in Molecular Biology (Clifton, N.J.)
|June 10, 2019
Summary
Peripheral blood mononuclear cells (PBMCs) are cultured to assess cell-mediated immunity and immune responses. This method measures lymphocyte proliferation upon antigen stimulation, aiding in allergy detection and immunotherapy monitoring.
Area of Science:
- Immunology
- Cell Biology
Background:
- Peripheral blood mononuclear cells (PBMCs) are crucial for evaluating cell-mediated immunity.
- PBMCs can detect prior antigen exposure and monitor immunotherapy effectiveness.
- In vitro culture of PBMCs allows for detailed immune response analysis.
Purpose of the Study:
- To describe the methodology for culturing T cells from peripheral blood.
- To outline the process of collecting cell supernatants for cytokine analysis.
- To provide a framework for assessing antigen-specific immune responses.
Main Methods:
- Isolation of PBMCs from peripheral blood.
- In vitro culture of T cells.
- Antigen-specific stimulation of PBMCs.
- Measurement of lymphocyte proliferation via (3H)-thymidine incorporation.
- Collection of cell supernatants for cytokine assays.
Main Results:
- PBMCs from previously exposed individuals proliferate upon specific antigen stimulation.
- Lymphocyte proliferation serves as a key indicator of immune response.
- The described methods enable the quantification of cellular immunity.
Conclusions:
- PBMC culture and stimulation assays are valuable tools for assessing cell-mediated immunity.
- These techniques aid in diagnosing allergies and monitoring immunotherapies.
- The chapter provides a practical guide for T cell culture and cytokine measurement.
Related Concept Videos
01:21Cell Culture
22.2K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
22.2K
01:20Protein Buffers in Blood Plasma and Cells
3.7K
The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
Certain amino acids can exist in a zwitterion state at a...
3.7K
01:47Primary Active Transport
197.1K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction...
197.1K
01:17Stem Cell Culture
6.0K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
6.0K
03:02Primary and Secondary Growth in Roots and Shoots
60.3K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.3K
01:24Peripheral Nervous System: Ganglia and Nerves
5.2K
The Peripheral Nervous System (PNS) is a crucial component of the body's neural network, extending beyond the central nervous system (CNS) to bridge the gap between the CNS and the external environment. It encompasses nerves, ganglia, and sensory receptors.
Nerves
The nerve is a bundle of axons that serves as the communication highway in the PNS. Each nerve is ensheathed in a protective layer of connective tissue called the epineurium. This outermost layer safeguards the nerve and supports the...
Nerves
The nerve is a bundle of axons that serves as the communication highway in the PNS. Each nerve is ensheathed in a protective layer of connective tissue called the epineurium. This outermost layer safeguards the nerve and supports the...
5.2K