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Updated: Jan 23, 2026

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
Published on: May 8, 2016
Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies
Nadine E G Upton1,2, Thomas S Hayday3, Louisa K James4
1Asthma and Allergy, Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.
Abstract:
The important role of the local mucosal environment in both the initiation and progression of allergic disease is well established. Analysis of tissue-resident lymphocyte subsets by flow cytometry requires isolation of viable cells from mucosal samples.Here we describe an advanced method to dissociate lymphocytes from human mucosal (e.g., nasal, bronchial) biopsies. Single-cell suspensions are obtained through a combination of gentle mechanical disruption and incubation of tissue with proteolytic enzymes. This method fully utilizes limited clinical samples and is amenable to a variety of downstream applications for phenotypic, single-cell analysis of tissue lymphocytes or pooled lymphocyte subsets.
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