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Updated: Jan 23, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
Published on: January 7, 2022
Continuous flow microfluidic cell inactivation with the use of insulating micropillars for multiple electroporation
Sanam Pudasaini1, A T K Perera2, Dhiman Das3
1School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore.
Abstract:
Electroporation is a powerful tool for inactivating cells and transfecting biological cells and has applications in biology, genetic engineering, medicine, environment, and many others. We report a new continuous flow device embedded with insulating micropillars to achieve better performance of cell inactivation. The use of micropillars creates multiple electroporation zones with enhanced local electric field strengths. Using a model solution of Saccharomyces cerevisiae, we examined the inactivation performance of the device under various applied electric voltages and flow rates. Results from the numerical simulations and experiments showed that even with an induced transmembrane potential of 0.58 V, close to 63% of cell inactivation was achieved at a flow rate of 2.5 mL/h. This was higher than the 24% cell inactivation observed for a reference device without micropillars that was subjected to the same conditions.
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