Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

64.8K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.8K
PCR01:32

PCR

237.2K
Overview
237.2K
Noncompartmental Analysis: Mean Residence Time01:05

Noncompartmental Analysis: Mean Residence Time

594
According to statistical moment theory, mean residence time (MRT) is an important measure in pharmacokinetics. MRT can be defined as the expected mean of a probability density function distribution. It provides valuable insights into drug disposition in the body.
After the administration of a drug through intravenous bolus injection, the drug molecules are distributed throughout the body and remain there for varying periods. The MRT represents the average time these drug molecules stay in the...
594
Noncompartmental Analysis: Mean Transit, Absorption and Dissolution Time01:02

Noncompartmental Analysis: Mean Transit, Absorption and Dissolution Time

379
When drugs are administered extravascularly, a comprehensive evaluation through noncompartmental analysis becomes imperative. This analytical approach considers various parameters that play a crucial role in understanding the pharmacokinetics of these drugs.
One of the key parameters is the mean transit time (MTT), which refers to the total duration required for drug molecules to transit through the body. MTT is determined by calculating the ratio of the area under the moment curve to the area...
379
Generation Time01:22

Generation Time

1.4K
Bacterial generation time, the period required for a bacterial population to double during its exponential growth phase, serves as a critical measure of microbial growth dynamics under optimal conditions. This parameter varies significantly across bacterial species and can be influenced by factors such as temperature, pH, and the availability of nutrients. For example, Escherichia coli can achieve a generation time of approximately 20 minutes, while Mycobacterium tuberculosis exhibits a much...
1.4K
Mixing Time01:19

Mixing Time

467
The concept of mixing time is significant in producing a uniform concrete mix with the required strength. The mixing period starts once all components are in the mixer. Initially, the mixer is charged with 10% of the water, followed by the consistent addition of solids and then 80% of the water. The remaining water is added later, within the first quarter of the mixing period. The minimum mixing time varies according to the mixer's capacity; for example, mixers with up to 1 cubic yard...
467

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-Wide Association Study for Glucocorticoid-Induced Ocular Hypertension.

Ophthalmology science·2026
Same author

Trends in pediatric uveitis: A systematic review and meta-epidemiological investigation of geographic, economic, and climate factors.

Survey of ophthalmology·2026
Same author

Clinical Outcomes and Genomic Epidemiology of Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Keratitis.

American journal of ophthalmology·2026
Same author

Slow Thinking for Fast Results: Reclaiming Analytical Reasoning in Antimicrobial Susceptibility Test Interpretation.

Open forum infectious diseases·2026
Same author

Substance Use Disorder Interventions and Ophthalmic Outcomes for Injection Drug Use-Associated Endogenous Endophthalmitis.

Ophthalmology. Retina·2026
Same author

Genome-Wide Association Study for Glucocorticoid-Induced Ocular Hypertension.

medRxiv : the preprint server for health sciences·2026

Related Experiment Video

Updated: Jan 23, 2026

Primed Mycobacterial Uveitis PMU as a Model for Post-Infectious Uveitis
10:33

Primed Mycobacterial Uveitis PMU as a Model for Post-Infectious Uveitis

Published on: December 17, 2021

3.1K

Real-Time Multiplex PCR Analysis in Infectious Uveitis.

Caroline L Minkus1, Paulo J M Bispo1, George N Papaliodis1

  • 1a Massachusetts Eye and Ear Infirmary/Harvard Medical School , Boston , MA , USA.

Seminars in Ophthalmology
|June 11, 2019
PubMed
Summary
This summary is machine-generated.

Real-time multiplex PCR offers a fast and accurate method for diagnosing infectious uveitis, identifying pathogens like herpesviruses and Toxoplasma gondii. This molecular test aids in correct diagnosis and timely treatment, preventing vision loss.

Keywords:
Multiplex PCRinfectious uveitisocular inflammationpolymerase chain reactionuveitis diagnosis

More Related Videos

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
07:00

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

17.9K
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
06:25

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Published on: July 10, 2019

22.4K

Related Experiment Videos

Last Updated: Jan 23, 2026

Primed Mycobacterial Uveitis PMU as a Model for Post-Infectious Uveitis
10:33

Primed Mycobacterial Uveitis PMU as a Model for Post-Infectious Uveitis

Published on: December 17, 2021

3.1K
qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
07:00

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

17.9K
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
06:25

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Published on: July 10, 2019

22.4K

Area of Science:

  • Ophthalmology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Infectious uveitis can lead to severe vision loss and ocular damage.
  • Clinical diagnosis is challenging due to overlapping symptoms of common causes like herpesviruses and Toxoplasma gondii.
  • A rapid and definitive diagnostic method is needed to guide treatment.

Purpose of the Study:

  • To review the current state and clinical utility of real-time multiplex PCR for diagnosing infectious uveitis.
  • To assess the sensitivity and specificity of this molecular diagnostic technique.

Main Methods:

  • A comprehensive literature review was conducted.
  • Searches included terms like "real time multiplex PCR", "infectious uveitis", and "uveitis diagnosis".
  • Included were relevant English-language publications detailing the application of this technology.

Main Results:

  • Four research groups reported successful use of real-time multiplex PCR for infectious uveitis diagnosis.
  • The technique demonstrated high sensitivity and specificity in validation studies.
  • Molecular results corrected clinical misdiagnoses in some cases, enabling appropriate antimicrobial therapy and improving patient outcomes.

Conclusions:

  • Real-time multiplex PCR provides a rapid, sensitive, and specific method for identifying infectious agents in uveitis.
  • This molecular assay facilitates prompt and effective treatment, mitigating ocular damage and preserving vision.