Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sign Test for Median of Single Population01:20

Sign Test for Median of Single Population

351
In general, the sign test serves as a nonparametric method to test hypotheses about the median of a single population when the data does not follow a known distribution. This simplicity makes it particularly useful for small sample sizes or when the assumptions of parametric tests cannot be met. The process begins with identifying a null hypothesis, typically stating that the population median equals a specific value. The alternative hypothesis could be that the median is either not equal to,...
351
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.5K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.5K
Angular Momentum: Single Particle01:10

Angular Momentum: Single Particle

7.6K
Angular momentum is directed perpendicular to the plane of the rotation, and its magnitude depends on the choice of the origin. The perpendicular vector joining the linear momentum vector of an object to the origin is called the “lever arm.” If the lever arm and linear momentum are collinear, then the magnitude of the angular momentum is zero. Therefore, in this case, the object rotates about the origin such that it lies on the rim of the circumference defined by the lever arm...
7.6K
The Pauli Exclusion Principle03:06

The Pauli Exclusion Principle

59.1K
The arrangement of electrons in the orbitals of an atom is called its electron configuration. We describe an electron configuration with a symbol that contains three pieces of information:
59.1K
Principle of Linear Impulse and Momentum for a Single Particle01:20

Principle of Linear Impulse and Momentum for a Single Particle

1.6K
Linear momentum is a fundamental concept in physics that describes the motion of an object. It is a vector quantity, having a magnitude equal to the product of its mass and its velocity, and direction along the object's velocity. On the other hand, linear impulse, also known as momentum impulse, is a concept in physics related to the change in the linear momentum of an object. Impulse is a vector quantity defined as the product of force and the time over which the force is applied.
Delving...
1.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CXCL13-CXCR5 signaling in CD8<sup>+</sup> T cell recruitment and lymphoid immune organization in clear cell renal cell carcinoma.

Cancer immunology, immunotherapy : CII·2026
Same author

Diverse Bacterial Properties Influence Dispersal Along Fungal Networks.

Journal of fungi (Basel, Switzerland)·2026
Same author

Integrative Surface Antigen Profiling of KLK2 and STEAP1 in Advanced Prostate Cancer.

Molecular cancer research : MCR·2026
Same author

CXCL13-CXCR5 Signaling in CD8<sup>+</sup> T Cell Recruitment and Lymphoid Immune Organization in Clear Cell Renal Cell Carcinoma.

bioRxiv : the preprint server for biology·2026
Same author

NCCN Guidelines® Insights: Prostate Cancer, Version 5.2026.

Journal of the National Comprehensive Cancer Network : JNCCN·2026
Same author

A Head and Neck Cancer Patient-Specific Microphysiological System for Predicting Response to Chemoradiation.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jan 23, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.9K

Automated System for Small-Population Single-Particle Processing Enabled by Exclusive Liquid Repellency.

Chao Li1, David J Niles1, Duane S Juang1

  • 1Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.

SLAS Technology
|June 11, 2019
PubMed
Summary
This summary is machine-generated.

Exclusive liquid repellency (ELR) enables automated handling of rare cells. This new platform rapidly isolates, identifies, and retrieves single particles using microdrops for advanced biological applications.

Keywords:
exclusive liquid repellencymulti-liquid-phaseopen microfluidicssingle-particle processingunderoil

More Related Videos

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

6.7K
A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

15.1K

Related Experiment Videos

Last Updated: Jan 23, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.9K
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

6.7K
A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

15.1K

Area of Science:

  • Materials Science
  • Biotechnology
  • Microfluidics

Background:

  • Exclusive liquid repellency (ELR) is an extreme wettability phenomenon where liquid droplets are repelled from a solid surface by an immiscible liquid.
  • Previous work established an open microfluidic system utilizing ELR for rare-cell culture and single-cell processing.
  • The ELR system offers ultra-low sample loss and biofouling, ideal for handling limited rare cellular samples.

Purpose of the Study:

  • To develop an automated platform for single-particle and single-cell isolation, identification, and retrieval.
  • To leverage Exclusive Liquid Repellency (ELR) microdrops for enhanced sample handling.
  • To create a hands-free and robust system for rare cellular sample manipulation.

Main Methods:

  • Integration of a robotic liquid handler with an automated microscopic imaging system.
  • Development of real-time image-processing software for single-particle identification.
  • Utilization of Exclusive Liquid Repellency (ELR) microdrops for encapsulation and manipulation.

Main Results:

  • Successful demonstration of an automated platform for single-particle isolation, identification, and retrieval.
  • Achieved rapid, hands-free, and robust isolation of microdrop-encapsulated rare cellular samples.
  • Validated the efficiency of ELR microdrops in lossless manipulation of biological samples.

Conclusions:

  • The automated ELR platform provides a significant advancement for handling rare cellular samples.
  • This technology enables precise and efficient isolation and retrieval of single particles.
  • The developed system holds promise for various biological applications requiring sensitive sample manipulation.