Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

606
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
606
Compounds Essential to Human Function01:25

Compounds Essential to Human Function

10.1K
The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all physiological processes in the body and are broadly classified into organic and inorganic based on their chemical structures.
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
10.1K
Essential Minerals for Bone Health01:31

Essential Minerals for Bone Health

6.1K
The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
6.1K
The Roles of Bacteria and Fungi in Plant Nutrition02:11

The Roles of Bacteria and Fungi in Plant Nutrition

47.2K
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
47.2K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

9.0K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.0K
Gene Flow02:39

Gene Flow

37.6K
Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
37.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Butuanimides, Fatty Acid Synthesis-Inhibiting Antibiotics from Symbiotic Bacteria.

ACS chemical biology·2026
Same author

A Decade of Leadership and Impact: Celebrating 10 Years of the ORS Spine Section.

JOR spine·2026
Same author

Automated Annotation of Pain Chronicity in Patients With Back Pain by Using Electronic Health Records: Retrospective Study.

JMIR formative research·2026
Same author

The effect of spaceflight on tissues of the spinal column.

Bone reports·2026
Same author

Real-World Effect of a Digitally Delivered Conservative Musculoskeletal Care Program on Spinal Diagnostic Imaging Utilization in a Commercially Insured Population with Chronic Back Pain.

Journal of health economics and outcomes research·2025
Same author

Short tandem gene duplications as potential agents of genetic memory.

mBio·2025

Related Experiment Video

Updated: Jan 23, 2026

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes
08:13

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Published on: September 1, 2018

18.0K

Essential gene deletions producing gigantic bacteria.

Jeannie Bailey1, Julie Cass2, Joe Gasper1

  • 1Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America.

Plos Genetics
|June 11, 2019
PubMed
Summary

Eliminating peptidoglycan synthesis in Acinetobacter baylyi causes cells to become giant and lyse. This process involves specific enzymes like MltD and LdtG, conserved in related species.

More Related Videos

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
10:35

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Published on: January 30, 2020

7.2K
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
14:07

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Published on: November 23, 2012

17.0K

Related Experiment Videos

Last Updated: Jan 23, 2026

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes
08:13

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Published on: September 1, 2018

18.0K
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
10:35

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Published on: January 30, 2020

7.2K
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
14:07

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Published on: November 23, 2012

17.0K

Area of Science:

  • Microbiology
  • Cell Biology
  • Bacterial Pathogenesis

Background:

  • Peptidoglycan is essential for bacterial cell structure and integrity.
  • Disruptions in peptidoglycan synthesis can lead to cell death and morphological changes.

Purpose of the Study:

  • To investigate the consequences of impaired peptidoglycan synthesis on bacterial cell morphology.
  • To identify genetic factors influencing giant cell formation in Acinetobacter species.

Main Methods:

  • Generating deletion mutations in Acinetobacter baylyi via natural transformation.
  • Treating bacteria with antibiotics targeting peptidoglycan synthesis.
  • Utilizing genome-scale transposon sequencing (Tn-seq) for mutant screening.

Main Results:

  • Loss of peptidoglycan synthesis genes resulted in the formation of polymorphic giant cells.
  • Antibiotic treatment also induced giant cell formation, which eventually led to lysis.
  • Tn-seq identified MltD and LdtG as key enzymes regulating giant cell formation.

Conclusions:

  • Giant cell formation in Acinetobacter is a complex process involving specific enzymatic activities.
  • A conserved genetic pathway for giant cell formation exists in Acinetobacter species, including the pathogen A. baumannii.