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Pericentrin-mediated SAS-6 recruitment promotes centriole assembly.

Daisuke Ito1, Sihem Zitouni1, Swadhin Chandra Jana1

  • 1Instituto Gulbenkian de Ciência, Oeiras, Portugal.

Elife
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Summary

The pericentriolar material (PCM) recruits centriole assembly machinery, including SAS-6, via Pcp1/pericentrin interaction. This conserved mechanism is crucial for centriole elongation, even in organisms lacking centrioles.

Keywords:
D. melanogasterS. pombeSAS-6SPBcell biologycentriolecentrosomeevolutionpericentrin

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Structural Biology

Background:

  • Centrosomes comprise centrioles and pericentriolar material (PCM).
  • Centrioles regulate PCM assembly, but PCM's role in centriole assembly is unclear.
  • Fission yeast possesses a centrosome (SPB) with PCM but lacks centrioles.

Purpose of the Study:

  • Investigate the interaction between centriole components and PCM.
  • Determine the role of PCM in centriole assembly.
  • Explore conserved mechanisms of centriole biogenesis.

Main Methods:

  • Ectopic expression of animal centriole components in fission yeast.
  • Investigating protein-protein interactions between PCM and centriole components.
  • Analyzing the role of specific protein domains (e.g., calmodulin-binding region) in interactions.

Main Results:

  • Ectopic animal centriole components, like SAS-6, were recruited to the fission yeast SPB.
  • A conserved PCM component, Pcp1/pericentrin, interacts with and recruits SAS-6.
  • This Pcp1/pericentrin-SAS-6 interaction is essential for centriole assembly, particularly elongation.
  • The calmodulin-binding region of Pcp1/pericentrin is critical for SAS-6 interaction.

Conclusions:

  • PCM actively recruits centriole assembly machinery, not just microtubule nucleators.
  • This interaction promotes centriole biogenesis in proximity to the PCM.
  • Conserved mechanisms link PCM and centriole assembly, even in centriole-less organisms.