Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.7K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.7K
Protein Complex Assembly02:41

Protein Complex Assembly

16.7K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.7K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.4K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.4K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

8.4K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
8.4K
Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

5.3K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ASO Visual Abstract: Does the Lymph Node Dissection during Esophagectomy Impact the Long-term Prognosis for Complete Responders After Neoadjuvant Therapy? A Meta-analysis.

Annals of surgical oncology·2026
Same author

Systems-level proteomic models of cotton fiber development: a high-resolution data resource to analyze cell dynamics and trait engineering.

Plant physiology·2026
Same author

Does Lymph Node Dissection During Esophagectomy Impact the Long-Term Prognosis for Complete Responders After Neoadjuvant Therapy? A Meta-analysis.

Annals of surgical oncology·2026
Same author

Patient-Derived Lung Cancer Organoids for Precision Oncology: A Comprehensive Analysis.

JTO clinical and research reports·2026
Same author

STAT3-mediated transactivation of NOVA2 promotes lung adenocarcinoma metastasis by splicing SMAD4.

Oncogene·2026
Same author

Comparative multi "omics" profiling of <i>Gossypium hirsutum</i> and <i>Gossypium barbadense</i> fibers at high temporal resolution reveals key differences in polysaccharide composition and associated glycosyltransferases.

Frontiers in plant science·2026

Related Experiment Video

Updated: Jan 23, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.8K

A Label-free Mass Spectrometry Method to Predict Endogenous Protein Complex Composition.

Zachary McBride1, Donglai Chen2, Youngwoo Lee1

  • 1‡Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana.

Molecular & Cellular Proteomics : MCP
|June 13, 2019
PubMed
Summary

This study introduces a novel mass spectrometry method to predict protein complex composition using elution profiles. This approach aids in understanding cellular systems and protein interactions without relying on bioinformatics data.

Keywords:
ArabidopsisLabel-free quantificationMacromolecular complex analysisPlant Biology*Protein complex analysisProtein correlation profiling

More Related Videos

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.9K
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

5.1K

Related Experiment Videos

Last Updated: Jan 23, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.8K
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.9K
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

5.1K

Area of Science:

  • Biochemistry
  • Proteomics
  • Systems Biology

Background:

  • Protein complex composition is crucial for understanding cellular mechanisms and pathway regulation.
  • Many cytosolic proteins exist in complexes, but their composition remains largely unknown.
  • Existing methods for predicting protein complex subunits often rely on bioinformatics data, which has limitations.

Purpose of the Study:

  • To develop a mass spectrometry-based method for predicting protein complex composition using biochemical data alone.
  • To overcome the limitations of bioinformatics-dependent approaches for protein complex prediction.
  • To enable systems-level analyses of protein complex dynamics.

Main Methods:

  • Utilized mass spectrometry-based protein correlation profiling.
  • Separated protein extracts by size and charge under nondenaturing conditions.
  • Applied clustering analyses to reproducible elution profiles of over 1000 proteins.

Main Results:

  • Predicted the composition of hundreds of known and novel protein complexes.
  • Identified protein complexes likely to assemble through self-interaction.
  • Demonstrated the method's ability to drive protein complex discovery.

Conclusions:

  • The developed method accurately predicts protein complex composition from biochemical data.
  • This approach facilitates protein complex discovery and hypothesis testing.
  • Enables comprehensive systems-level analyses of protein complex dynamics across organisms.