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Transitional cell carcinoma: lymphocyte-mediated cytotoxicity
National Cancer Institute Monograph
|December 1, 1978
Summary
A new method effectively removes Fc-positive lymphocytes from human blood. The remaining cells show preserved cytotoxic activity against target cells, indicating a viable cell population for further study.
Area of Science:
- Immunology
- Cell Biology
Background:
- Fc-positive lymphocytes play a role in immune responses.
- Efficiently isolating specific lymphocyte subsets is crucial for immunological research.
Purpose of the Study:
- To present a method for the selective removal of Fc-positive lymphocytes from human peripheral blood.
- To evaluate the functional capacity of the remaining lymphocyte populations.
Main Methods:
- Development and application of a method to isolate Fc-positive lymphocytes.
- Assessment of the cytotoxic activity of the resultant cell population against autochthonous and allogeneic target cells.
Main Results:
- Successful depletion of Fc-positive lymphocytes from peripheral blood.
- Demonstration of retained cytotoxic function in the remaining cell population against both self and non-self target cells.
Conclusions:
- The presented method allows for the effective isolation of Fc-negative lymphocytes.
- The isolated lymphocyte population maintains its cytotoxic potential, making it suitable for immunological investigations.