Application of hydroxyapatite nanoparticle-assembled powder using basic fibroblast growth factor as a pulp-capping...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Dental Research

Background:

• Hydroxyapatite (HAP) nanoparticle-assembled powder (nano-HAP) can be fabricated without high-temperature or pressure treatments.
• Fibroblast growth factor 2 (FGF2) is known to promote odontoblast differentiation.

Purpose of the Study:

• To evaluate nano-HAP combined with FGF2 as a pulp-capping agent for dentin defects.
• To assess the biocompatibility and hard tissue regenerative potential of nano-HAP/FGF2 in rat molars.

Main Methods:

• Dentin defects were created in rat molars and capped with nano-HAP/FGF2.
• Tissue response was evaluated radiologically and histologically at 1 and 2 weeks post-capping.

Main Results:

• The nano-HAP/FGF2 combination induced dental pulp cell and vessel invasion.
• Consistent formation of a dentinal bridge with numerous tubules was observed.
• The material demonstrated biocompatibility and stimulated hard tissue formation.

Conclusions:

• Nano-HAP/FGF2 serves as an effective physiological approach for promoting pulp tissue regeneration.
• This biomaterial combination successfully treated pulp exposure by stimulating dentinal bridge formation.
• Further research is needed to elucidate the precise mechanisms of calcified tissue formation by nano-HAP/FGF2.