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Updated: Jan 23, 2026

Revised and Neuroimaging-Compatible Versions of the Dual Task Screen
Published on: October 5, 2020
Dual inhibitors of LSD1 and spermine oxidase
Steven Holshouser1, Matthew Dunworth2, Tracy Murray-Stewart2
1Department of Drug Discovery and Biomedical Sciences , Medical University of South Carolina , 70 President St. , Charleston , SC 29425 , USA .
Abstract:
We have previously described the synthesis and evaluation of 3,5-diamino-1,2,4-triazole analogues as inhibitors of the flavin-dependent histone demethylase LSD1. These compounds are potent inhibitors of LSD1 without activity against monoamine oxidases A and B, and promote the elevation of H3K4me2 levels in tumor cells in vitro. We now report that the cytotoxicity of these analogues in pancreatic tumor cells correlates with the overexpression of LSD1 in each tumor type. In addition, we show that a subset of these 3,5-diamino-1,2,4-triazole analogues inhibit a related flavin-dependent oxidase, the polyamine catabolic enzyme spermine oxidase (SMOX) in vitro.
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