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Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Published on: July 27, 2022
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In vitro studies on gelatin/hydroxyapatite composite modified with osteoblast for bone bioengineering
Namrata Yadav1, Pradeep Srivastava1
1School of Biochemical Engineering, IIT (BHU), Varanasi, 221005, India.
Heliyon
|June 14, 2019
Summary
Human mesenchymal stem cell-derived bone cells show potential for bone tissue engineering. These cells, when cultured on gelatin/hydroxyapatite/chitosan scaffolds, offer a promising co-culture system for bone regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Developing effective bone tissue engineering strategies requires biomaterials that mimic the extracellular matrix.
- Investigating optimal cell sources and scaffold combinations is crucial for successful bone regeneration.
Purpose of the Study:
- To synthesize and characterize gelatin/hydroxyapatite/chitosan composites for bone tissue engineering.
- To evaluate the suitability of human mesenchymal stem cell-derived bone cells in a co-culture system on these scaffolds.
Main Methods:
- Composite synthesis and characterization using SEM-EDX, XRD, FTIR, and porosity measurements.
- In vitro assessment of biodegradation (lysozyme) and biomineralization (SBF).
- In vitro cell studies using flow cytometry, CLSM, SEM, and DSC to evaluate cell behavior.
Main Results:
- Gelatin/hydroxyapatite/chitosan composites were successfully synthesized and characterized.
- In vitro studies demonstrated favorable biodegradation and biomineralization properties.
- Human mesenchymal stem cell-derived bone cells exhibited good viability and integration within the composite scaffolds.
Conclusions:
- The developed gelatin/hydroxyapatite/chitosan composite scaffolds support human bone cell growth and function.
- Human mesenchymal stem cell-derived bone cells represent a promising cell source for bone regenerative applications.
- This co-culture system offers a viable strategy for bone tissue engineering.
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