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Clinically Deployable Electro-Immunosensing Device Comprising Bioactive Glass-MWCNT for Alkaline Phosphatase
Darshna1, Indrani Nandi1, Pradeep Srivastava1
1School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
ACS Applied Bio Materials
|January 10, 2025
Summary
A novel biosensor using bioactive glass and carbon nanotubes detects alkaline phosphatase (ALP) in serum. This label-free electrochemical impedance spectroscopy method offers high sensitivity and selectivity for clinical bone disease evaluation.
Area of Science:
- Biomaterials Science
- Biosensor Technology
- Analytical Chemistry
Background:
- Bone is a dynamic tissue crucial for calcium storage, hematopoiesis, and structural support.
- Alkaline phosphatase (ALP), secreted by osteoblasts, is a key biomarker for bone metabolism and disease.
- Current methods for ALP detection may require labels or lack sufficient sensitivity for clinical diagnostics.
Purpose of the Study:
- To develop a novel, label-free biosensor for detecting alkaline phosphatase (ALP).
- To utilize a composite probe of bioactive glass (BG) and multiwalled carbon nanotubes (MWCNT) for enhanced detection.
- To evaluate the biosensor's analytical performance and selectivity in biological samples.
Main Methods:
- Fabrication of a composite probe using bioactive glass (BG) and multiwalled carbon nanotubes (MWCNT).
- Characterization of the biosensor probe through physical methods and electrochemical analysis.
- Evaluation of analytical performance using electrochemical impedance spectroscopy (EIS), including limit of detection (LOD) and linear dynamic range (LDR).
- Assessment of selectivity against serum interfering chemicals and validation in human and fetal bovine serum samples.
Main Results:
- The BG/MWCNT composite biosensor demonstrated label-free detection of ALP via EIS.
- Achieved a limit of detection (LOD) of 2.29 ± 0.35 U/L and a linear dynamic range (LDR) of 25-600 U/L.
- Exhibited high selectivity for ALP with a selectivity coefficient (Ksel) < 0.06.
- Successfully detected ALP in both human serum and fetal bovine serum samples.
Conclusions:
- The developed BG/MWCNT composite biosensor is a promising tool for sensitive and selective ALP detection.
- This label-free electrochemical approach offers a viable alternative for clinical evaluation of bone-related disorders.
- The biosensor's performance characteristics are suitable for routine clinical diagnostics and biological sample analysis.
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