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An efficient method for measuring plasma volume using indocyanine green dye.

Sixtus Aguree1, Alison D Gernand1

  • 1Department of Nutritional Sciences, The Pennsylvania State University, 110 Chandlee Laboratory, University Park, PA, 16802, United States.

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Summary
This summary is machine-generated.

This study presents an optimized protocol using Indocyanine green (ICG) to efficiently measure plasma volume (PV). The refined method simplifies plasma volume determination, making it more accessible for health status assessment.

Keywords:
Blood volumeIndocyanine greenMeasuring plasma volume using ICGNonpregnant womenNutritional biomarkerPlasma volume

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Area of Science:

  • Physiology
  • Biomarker Analysis
  • Medical Diagnostics

Background:

  • Plasma volume (PV) is a critical health indicator but rarely measured due to complex, time-consuming methods.
  • Indocyanine green (ICG) is a validated dye for PV measurement, yet detailed protocols are scarce.

Purpose of the Study:

  • To develop and detail an optimized protocol for measuring plasma volume (PV) using Indocyanine green (ICG).
  • To enhance efficiency and reduce participant burden in PV determination for women of reproductive age.

Main Methods:

  • Utilized a refined protocol combining best practices for ICG administration and plasma sampling.
  • Collected five post-injection plasma samples within 2-5 minutes after ICG bolus for decay curve analysis.
  • Minimized plasma requirement to 2.5 mL and utilized a single IV catheter for participant convenience.

Main Results:

  • Reduced the time for plasma volume determination to approximately 2 hours.
  • Established an efficient method requiring only 2.5 mL of plasma for accurate PV estimation.
  • Simplified the procedure by using a single intravenous catheter, reducing participant inconvenience.

Conclusions:

  • The optimized ICG protocol provides a rapid, efficient, and less invasive method for plasma volume measurement.
  • This streamlined approach facilitates broader application of PV assessment in clinical and research settings.
  • Accurate PV determination is achievable with minimal plasma and reduced procedural time.