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Related Experiment Video

Updated: Jul 7, 2026

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Published on: March 23, 2022

Optimized protocols for culturing and sectioning mouse intestinal organoids: enhancing efficiency and structural

Jiawei Li¹, Rong Jin¹, Xuanxuan Zhang²

¹International Joint Research Center of Stem Cell Bank, Ministry of Science and Technology, Northeast Normal University, Changchun, 130117, China.

|July 6, 2026

Summary

This study presents an optimized protocol for mouse intestinal organoid culture and histological preparation. The new method reduces processing time and sample loss, improving the quality of 3D intestinal models for research.

Keywords:

Crypt Embedding and sectioning Organoids

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The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

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Last Updated: Jul 7, 2026

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Published on: March 23, 2022

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

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Published on: April 7, 2023

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Published on: May 18, 2016

Area of Science:

Gastroenterology
Cell Biology
Histology

Background:

Mouse intestinal organoids are vital 3D models for studying intestinal biology due to their genetic fidelity and complex structure.
Traditional methods for organoid processing are complex and lead to sample loss during sectioning.
Maintaining the structural integrity of delicate crypt-villus morphology is challenging.

Purpose of the Study:

To develop an optimized protocol for establishing mouse intestinal organoid cultures.
To refine histological processing for enhanced immunohistochemistry and fluorescence staining.
To reduce operational complexity and sample loss in organoid-based research.

Main Methods:

A direct pellet-OCT embedding strategy was implemented to minimize organoid transfer and sample loss.
The workflow was optimized to reduce overall operational time.
Comparative evaluation with traditional agarose-paraffin methods was performed.

Main Results:

The optimized protocol enables high-quality frozen sections suitable for various staining techniques (H&E, Alcian Blue, IHC, IF).
The direct pellet-OCT embedding significantly reduces sample handling and associated loss.
Experimental quality and structural integrity of organoid morphology were enhanced.

Conclusions:

This protocol offers a practical and efficient alternative to conventional workflows for mouse intestinal organoid histology.
The method preserves delicate organoid morphology and simplifies processing.
The optimized approach facilitates high-quality histological analysis for intestinal biology research.