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Updated: Jan 23, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
A highly sensitive label-free electrochemical immunosensor based on poly(indole-5-carboxylicacid) with ultra-high
Taotao Yang1, Xiaoning Ren1, Ming Yang1
1College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan 430074, China.
Abstract:
The high stability of redox signal is one of the most crucial factors in construction of electrochemical immunosensors. However, the redox-active species usually show low stability and poor conductivity, which inhibits their application in electrochemical immunosensors. In this work, we report that the conductive polymer poly(indole-5-carboxylic acid) (PIn-5-COOH) possesses ultra-high redox stability. The redox signal of PIn-5-COOH could remain 96.03% after 500 cyclic voltammery (CV) cycles in buffer solution with pH of 6.2, while the redox signals in most of the previous reports only remained less than 90% after 50 CV cycles. Our mechanism investigation indicated that the ultra-high redox stability of PIn-5-COOH should be attributed to its stable structure. The electrochemical immunosensors fabricated with PIn-5-COOH/MWCNTs-COOH nanocomposite showed a wide linear range from 0.001 ng mL-1 to 100 ng mL-1 and a low detection limit of 0.33 pg mL-1 for the detection of alpha fetoprotein. This study opens up a new avenue for the construction of electrochemical immunosensors with ultra-stable redox signal.
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