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Updated: Jan 23, 2026

A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
Published on: October 11, 2018
Lack of Correlation between the Enterotoxigenicity of Clostridium perfringens Isolates and Propionic Acid Formation
Ronald G Labbe1, Kyung-Ryoul Lee1, Patrick L Perry1
1Food Microbiology Laboratory, Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Abstract:
The absence in the supernatant fluid of either extracellular amylase in cultures grown at 46°C or propionic acid has been proposed for the presumptive identification of enterotoxin-positive strains of Clostridium perfringens . However we found that propionic acid was formed by several enterotoxin-positive, as well as enterotoxin-negative, strains of this organism. In addition 4 of 13 ent+ stains produced extracellular amylase during growth at 46°C. These results indicate that neither trait can be used as a reliable indicator of the enterotoxigenicity of isolates of this organism.
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