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Identification and Differentiation of Coagulase-Negative Staphylococcus aureus by Polymerase Chain Reaction

K R Matthews1, J Roberson2, B E Gillespie2

  • 1Department of Food Science, Rutgers University, New Brunswick, New Jersey 08903.

Journal of Food Protection
|June 15, 2019
PubMed

Insights

This study introduces a rapid and accurate polymerase chain reaction DNA fingerprinting method to distinguish coagulase-positive Staphylococcus aureus from coagulase-negative strains, improving diagnostic reliability.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Staphylococcus aureus is a common cause of infections in humans and animals.
  • Most S. aureus strains produce coagulase, but atypical coagulase-negative strains exist.
  • Accurate identification of S. aureus strains is crucial for effective treatment and control.

Purpose of the Study:

  • To develop and evaluate a polymerase chain reaction (PCR)-based DNA fingerprinting method.
  • To differentiate coagulase-positive Staphylococcus aureus (CPSA) from coagulase-negative Staphylococcus aureus (CNSA).

Main Methods:

  • Utilized PCR-based DNA fingerprinting technique.
  • Evaluated 50 CPSA and 29 CNSA isolates.
  • Assessed differentiation based on visible observation and densitometric analysis.

Main Results:

  • PCR-based DNA fingerprinting successfully differentiated CNSA from CPSA.
  • The method provided clear distinctions through visual and densitometric evaluation.
  • Demonstrated rapidity and accuracy compared to conventional methods.

Conclusions:

  • PCR-based DNA fingerprinting is a reliable method for distinguishing CPSA from CNSA.
  • This technique offers a rapid and accurate alternative to traditional identification methods.
  • Improved diagnostic accuracy can lead to better treatment and control strategies for S. aureus infections.
Keywords:
coagulase testidentificationpolymerase chain reaction

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