Related Experiment Video

Updated: Jan 23, 2026

Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

4.1K

Differential expression profiles and functional analysis of circular RNAs in children with fulminant myocarditis

Li Zhang1, Bo Han1, Jing Wang1

  • 1Department of Pediatric Cardiology, Shandong Provincial Hospital affiliated to Shandong University, No. 324 Jingwuweiqi Road, Jinan, Shandong 250021, PR China.

Epigenomics
|June 15, 2019
PubMed

Insights

Circular RNAs (circRNAs) show altered expression in pediatric fulminant myocarditis. The circRNA hsa_circ_0071542 may serve as a key biomarker for this condition.

Area of Science:

  • Molecular Biology
  • Pediatric Cardiology
  • Immunology

Background:

  • Fulminant myocarditis in children is a severe condition with complex underlying mechanisms.
  • Circular RNAs (circRNAs) are emerging as critical regulators in various biological processes, including inflammation and immunity.

Purpose of the Study:

  • To investigate the differential expression profiles of circRNAs in pediatric fulminant myocarditis.
  • To explore the potential functions and molecular interactions of these circRNAs.

Main Methods:

  • circRNA microarray analysis was performed on samples from children with fulminant myocarditis and healthy controls.
  • Bioinformatic analyses, including functional enrichment and network construction, were utilized to understand circRNA roles.

Main Results:

  • A significant number of circRNAs were found to be differentially expressed, with 2281 upregulated and 892 downregulated.
  • The circRNA hsa_circ_0071542 was notably upregulated (2.5-fold) in fulminant myocarditis patients.
  • Functional analyses indicated that differentially expressed circRNAs are primarily involved in inflammatory and immune responses.

Conclusions:

  • circRNAs play a significant role in the pathogenesis of pediatric fulminant myocarditis.
  • hsa_circ_0071542 presents potential as a diagnostic biomarker for pediatric fulminant myocarditis.

Keywords:
circular RNAfulminant myocarditisfunctional analysishsa_circ_0071542microarray

More Related Videos

Use of Alu Element Containing Minigenes to Analyze Circular RNAs13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.7K
Identification of Circular RNAs using RNA Sequencing08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.7K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.5K
Myocarditis I: Introduction01:21

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...
391
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.6K
Myocarditis IV: Nursing Management01:22

Myocarditis IV: Nursing Management

Myocarditis is an inflammatory condition of the myocardium requiring meticulous nursing management for optimal patient outcomes. Effective management begins with a thorough assessment of the patient's medical history, paying close attention to past infections, autoimmune disorders, travel history, and exposure to toxins or drugs. Recent viral infections and systemic diseases are particularly relevant due to their potential role in triggering myocarditis.Physical Examination and MonitoringThe...
225
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
195.1K