Related Experiment Videos
Strain difference in an allergic asthma model in rats.
M Misawa1, K Takenouchi, T Abiru
1Department of Applied Pharmacology, School of Pharmacy, Hoshi University, Tokyo, Japan.
Japanese Journal of Pharmacology
|September 1, 1987
Summary
Researchers developed a new rat asthma model to test antiallergic drugs. Lewis rats showed significant responses and were most suitable for studying agents like disodium cromoglycate (DSCG).
Area of Science:
- Pharmacology
- Immunology
- Respiratory Medicine
Background:
- Asthma models are crucial for understanding allergic responses.
- Evaluating antiallergic agents requires reliable animal models.
Purpose of the Study:
- To develop and validate a novel rat asthma model.
- To compare allergic bronchoconstrictor responses in different rat strains.
- To assess the efficacy of disodium cromoglycate (DSCG) in this model.
Main Methods:
- Active sensitization of Wistar, Lewis, and Fischer 344 rats with DNP-Ascaris antigen and Bordetella pertussis vaccine.
- Provocation of asthmatic response via DNP-Ascaris inhalation.
- Measurement of bronchomotor response using a modified Konzett-Rössler method.
- Intravenous administration of disodium cromoglycate (DSCG) to assess inhibition.
Main Results:
- A new rat asthma model successfully induced allergic bronchoconstriction without systemic effects.
- Bronchoconstriction severity varied: Wistar > Lewis = Fischer 344.
- Disodium cromoglycate (DSCG) inhibited bronchoconstriction by 28-36%, with significant effects in Lewis and Fischer 344 rats.
- Fischer 344 rats exhibited higher susceptibility to surgical damage.
Conclusions:
- Lewis rats are the most suitable strain for a rat asthma model to study antiallergic agents.
- The developed model effectively mimics allergic asthma responses.
- Disodium cromoglycate demonstrates efficacy in mitigating asthma-like symptoms in this model.