Related Experiment Video
Updated: Aug 9, 2026

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
Published on: February 22, 2018
20-Hydroxyecdysone increases levels of transient gene expression in transfected Drosophila cells
1Department of Medicine, Royal Postgraduate Medical School, London, UK.
Abstract:
Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, in situ, this increase appears to be due to an increase in the number of cells expressing the transfected DNA. The stimulation in levels of expression is not correlated to any specific DNA sequence, nor does it occur if cells are exposed to hormone post-transfection.
Related Concept Videos
Position-effect Variegation
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...

