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Updated: Jan 23, 2026

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
Published on: March 12, 2015
Rapid multi-element characterization of microgreens via total-reflection X-ray fluorescence (TXRF) spectrometry
Ignazio Allegretta1, Concetta Eliana Gattullo1, Massimiliano Renna2
1Department of Soil, Plant and Food Sciences, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy.
Abstract:
Microgreens are an emerging class of vegetables, which have become increasingly important in the agri-food market in recent years, and contain a number of macro- and micro-nutrients. This paper presents a rapid method for the elemental analysis of microgreens based on total reflection X-ray fluorescence (TXRF) spectroscopy, without preliminary sample digestion. The following elements were detected and quantified simultaneously for six microgreen genotypes, belonging to Asteraceae and Brassicaceae: P, S, K, Ca, Cl, Mn, Fe, Ni, Cu, Zn, Br, Rb, Sr. The limit of detection (LOD) varied depending on the element and ranged between 0.1 mg kg-1 for Sr and 42 mg kg-1 for P. The method was validated using certified standards, and results compared with those obtained using a conventional ICP-AES method requiring sample digestion. The paper also presents the advantages and disadvantages of the two techniques.
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