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Electrochemical paper-based microfluidic device for high throughput multiplexed analysis.

Elson Luiz Fava1, Tiago Almeida Silva2, Thiago Martimiano do Prado1

  • 1Department of Chemistry, Federal University of São Carlos, São Carlos, P.O. Box 676, 13560-970, SP, Brazil.

Talanta
|June 17, 2019
PubMed
Summary
This summary is machine-generated.

A novel disposable electrochemical paper-based device offers multiplexed analysis using sixteen channels, avoiding costly wax printers. This high-throughput platform enables rapid, safe clinical tests, including non-invasive glucose monitoring in urine.

Keywords:
Electrochemical paper-based analysisGlucoseMicrofluidicMultiplex analysisUrine

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Area of Science:

  • Electrochemistry
  • Biosensors
  • Microfluidics

Background:

  • Microfluidic electrochemical paper-based devices (EPDs) are valuable for point-of-care diagnostics.
  • Traditional EPD fabrication often relies on expensive wax printing methods.
  • Multiplexed analysis on EPDs requires efficient strategies for electrode integration.

Purpose of the Study:

  • To develop a cost-effective, disposable microfluidic electrochemical paper-based device for multiplexed analysis.
  • To demonstrate a novel approach for multiplexing working and reference electrodes using a commercial module.
  • To validate the device's performance for high-throughput clinical applications, including glucose detection.

Main Methods:

  • Fabrication of a 16-channel microfluidic electrochemical paper-based device without wax printing.
  • Utilizing a commercial multiplexing module for simultaneous operation of multiple channels.
  • Employing various electrochemical techniques (cyclic voltammetry, square-wave voltammetry, etc.) for device characterization.
  • Constructing a glucose biosensor using glucose oxidase for urine glucose determination.

Main Results:

  • The device successfully achieved multiplexed electrochemical detection across sixteen independent channels.
  • Demonstrated the feasibility of multiplexing both working and reference electrodes for the first time with the commercial module.
  • Achieved good repeatability and reproducibility for high-throughput analysis.
  • The glucose biosensor exhibited a linear response in the range of 1.0 × 10⁻⁴ to 4 × 10⁻² mol/L with a limit of detection of 3 × 10⁻⁵ mol/L.

Conclusions:

  • The proposed device offers a scalable, cost-effective, and user-friendly platform for multiplexed electrochemical analysis.
  • This innovative sensing technology facilitates rapid and safe clinical testing.
  • The non-invasive glucose monitoring in urine demonstrates the potential for point-of-care diagnostics.