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ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
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Electrochemical enzyme-linked oligonucleotide array for aflatoxin B1 detection.
Giulia Selvolini1, Mariagrazia Lettieri1, Luca Tassoni2
1Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy.
Talanta
|June 17, 2019
Summary
This study introduces a rapid electrochemical assay for detecting aflatoxin B1 (AFB1). The novel method uses an enzyme-linked oligonucleotide array for sensitive and simple multidetection of this mycotoxin.
Area of Science:
- Analytical Chemistry
- Biosensors
- Food Safety
Background:
- Aflatoxin B1 (AFB1) is a dangerous mycotoxin requiring sensitive detection methods.
- Existing detection methods can be complex and time-consuming.
- Development of rapid, simple, and sensitive biosensors is crucial for food safety.
Purpose of the Study:
- To develop a simple and rapid electrochemical enzyme-linked oligonucleotide array for multidetection of AFB1.
- To immobilize AFB1 and use a competitive assay format for enhanced sensitivity.
- To validate the assay's performance and applicability in real food samples.
Main Methods:
- Fabrication of disposable screen-printed cells (SPCs) with poly(aniline-anthranilic acid) copolymer.
- Immobilization of Aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on the modified electrodes.
- Competitive assay using biotinylated DNA-aptamer, streptavidin-alkaline phosphatase, and differential pulse voltammetry (DPV) detection.
Main Results:
- The electrochemical assay demonstrated a signal-off response characteristic of a competitive format.
- A linear dose-response curve was achieved for AFB1 detection in the range of 0.1–10 ng/mL.
- A low limit of detection (LOD) of 0.086 ng/mL was obtained, indicating high sensitivity.
Conclusions:
- The developed electrochemical enzyme-linked oligonucleotide array offers a simple, rapid, and sensitive method for AFB1 detection.
- The assay shows promise for on-site monitoring and ensuring food safety.
- Preliminary validation in spiked maize flour samples demonstrates practical applicability.
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