A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. colil-asparaginase

Area of Science:

• Biotechnology
• Analytical Chemistry
• Pharmaceutical Science

Background:

• Biosensors are crucial for pharmaceutical research and quality control.
• Surface Acoustic Wave (SAW) biosensors detect mass and viscosity changes via acoustic waves.
• SAW biosensors enable quantification of molecular binding events.

Purpose of the Study:

• To develop and validate a SAW biosensor for the functional quantification of E. coli L-asparaginase.
• To establish an efficient antibody immobilization strategy for the biosensor.
• To assess the method's suitability for pharmaceutical quality control.

Main Methods:

• Utilized a SAW biosensor with immobilized polyclonal antibodies against E. coli L-asparaginase.
• Optimized antibody immobilization using BS3 activated amide coupling via Protein G.
• Validated the method for selectivity, linearity, accuracy (102.4% recovery), and precision (8.5% RSD).

Main Results:

• The developed SAW biosensor method accurately quantified E. coli L-asparaginase.
• The method demonstrated suitability for analyzing various E. coli L-asparaginase samples, including different lots and heat-treated variants.
• The BS3 activated amide coupling via Protein G proved to be an effective immobilization strategy.

Conclusions:

• The validated SAW biosensor method is suitable for the functional quantification of E. coli L-asparaginase.
• This SAW biosensor approach can be adopted as a pharmaceutical quality control method.
• The study highlights the potential of SAW biosensors in pharmaceutical analysis.