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Coconut Water as an Extender Component for Cooled Equine Sperm
Lucas S Brasileiro1, Lorenzo Garrido Teixeira M Segabinazzi2, Erika Menezes1
1Department of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil.
Journal of Equine Veterinary Science
|June 18, 2019
Summary
Coconut water extenders did not preserve equine sperm motility during cooling. Formulations with powdered coconut water or 5% egg yolk significantly reduced sperm kinetics compared to a commercial extender, though plasma membrane integrity remained unaffected.
Area of Science:
- Reproductive Biology
- Animal Science
- Veterinary Medicine
Background:
- Effective preservation of equine sperm during cooling is crucial for artificial insemination programs.
- Novel extender components are continuously explored to improve sperm cryopreservation and cooling efficiency.
Purpose of the Study:
- To investigate the efficacy of powdered coconut water in different extender formulations for cooling equine sperm.
- To evaluate the impact of coconut water-based extenders on sperm kinetics and plasma membrane integrity.
Main Methods:
- Equine sperm were diluted in five extenders: powdered coconut water (ACP-105), ACP-105 with skimmed milk, ACP-105 with 2.5% or 5% egg yolk, and a commercial extender (BotuSêmen).
- Sperm were cooled at 5°C and 15°C for 24 hours in a passive cooling device.
- Sperm kinetics (total motility, progressive motility, rapid movement) and plasma membrane integrity were assessed before and after cooling.
Main Results:
- Sperm kinetics were significantly lower in ACP-105 and ACP-EY 5% extenders compared to BotuSêmen after 24 hours of cooling.
- No significant differences in sperm kinetics were observed at T0 (before cooling) among the groups.
- Plasma membrane integrity remained similar across all extender groups and cooling temperatures (5°C and 15°C).
Conclusions:
- Coconut water-based extenders, as formulated in this study, are not suitable alternatives for preserving equine sperm motility during cooling.
- Further research is needed to explore different compositions of coconut water in sperm extenders for equine use.
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