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Inactivation and Regeneration Kinetics of Horseradish Peroxidase Heated at High Temperatures
Carmen Rodrigo1, Miguel Rodrigo2, Andrés Alvarruiz3
1Departamento de Medicina Preventiva, Salud Pública, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universitat de València, C/Vicente Andres Estelles s/n, 46100 Burjassot, Valencia.
Abstract:
The inactivation kinetics of horseradish peroxidase (HRP) heated in capillary tubes in the range 110 to 135°C was studied. Its regeneration kinetics when stored at 4 and 25°C was also considered. As the severity of the treatment increased, the absolute value of the regeneration decreased. The storage temperature of the enzyme did not affect the percentage of maximum activity regenerable, although when this temperature was raised from 4 to 25°C the speed of regeneration increased. Kinetics of HRP inactivation determined after heating and after regeneration were compared. Both forms of the enzyme showed similar behavior with first-order inactivation kinetics, with E = 19.5 ± 1.0 kcal/mol and k120°C = 3.7 ± 0.2 min-1, and E = 18.8 ± 1.2 kcal/mol and k120°C = 3.7 ± 0.2 min-1, respectively.
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