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Colony stimulating factor (CSF) activity in human and murine sera.
Archivum Immunologiae Et Therapiae Experimentalis
|January 1, 1978
Summary
Researchers identified specific molecular weight fractions in serum exhibiting colony-stimulating factor (CSF) activity. This activity increased with endotoxin stimulation in mice, indicating a role in immune response.
Area of Science:
- Biochemistry
- Immunology
- Hematology
Background:
- Colony-stimulating factors (CSFs) are crucial for hematopoiesis and immune responses.
- Understanding the molecular nature and regulation of CSFs is vital for clinical applications.
Purpose of the Study:
- To characterize substances with colony-stimulating factor (CSF) activity in human and murine sera.
- To investigate the modulation of CSF activity by endotoxin stimulation.
Main Methods:
- Serum samples from humans and mice were analyzed using gel filtration chromatography on Sephadex G-200.
- CSF activity was assessed using a CSF test.
- Sensitivity to pepsin was evaluated.
Main Results:
- Two active fractions with molecular weights of 14,000 D and 6000 D were identified in sera.
- Endotoxin stimulation in mice resulted in a twofold increase in CSF activity for both fractions.
- A high molecular weight protein peak (300,000 D) also showed CSF activity upon endotoxin stimulation.
- CSF-active fractions demonstrated sensitivity to pepsin digestion.
Conclusions:
- Serum contains distinct molecular entities possessing CSF activity.
- Endotoxin significantly enhances CSF activity, suggesting a role in the acute phase response.
- The pepsin sensitivity indicates the proteinaceous nature of these CSF-active substances.