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Albumins and globulins in developing maize grains
1Laboratoire de Chimie biologique et de photophysiologie, INRA, Centre GMP, Thiverval-Grignon, France.
Biochimie
|June 1, 1987
Summary
This study examined protein changes in normal and opaque-2 maize grain development. Opaque-2 maize showed more significant albumin and globulin variations, impacting grain composition.
Area of Science:
- Plant Biochemistry
- Maize Genetics
- Protein Chemistry
Background:
- Albumins and globulins are key seed storage proteins in maize.
- Understanding their developmental dynamics is crucial for crop improvement.
- Opaque-2 (o2) mutations affect maize endosperm protein synthesis.
Purpose of the Study:
- To quantitatively and qualitatively analyze albumin and globulin changes during normal and o2 maize grain development.
- To compare protein dynamics between normal and o2 maize varieties.
- To discuss the extraction methodology and physiological roles of these proteins.
Main Methods:
- Sequential protein extraction using water and 0.5 M NaCl.
- Isolation of proteins via trichloroacetic acid salting out.
- Analysis of amino acid composition of extracted albumins and globulins.
Main Results:
- Albumins peaked at mid-development and then declined in both maize types.
- Globulins increased from near absence to maturity, with greater changes in o2 maize.
- Amino acid profiles remained relatively stable throughout grain development.
Conclusions:
- Developmental patterns of albumins and globulins differ between normal and o2 maize.
- The opaque-2 mutation influences the accumulation of these seed storage proteins.
- Sequential extraction methods provide insights into protein dynamics and physiological roles.