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Updated: Jan 23, 2026

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Published on: September 23, 2022
Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro
Xiaofang Cui1,2, Ping Qian3,4,5,6, Tingting Rao7,8
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. cuixiaofang@webmail.hzau.edu.cn.
Interleukin enhancer-binding factor 2 (ILF2) interacts with Japanese encephalitis virus (JEV) protein NS3. Lower ILF2 levels enhance JEV viral propagation, suggesting ILF2 as a potential antiviral target.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Japanese encephalitis virus (JEV) causes viral encephalitis.
- JEV NS3 protein is crucial for viral replication.
Purpose of the Study:
- Identify novel cellular proteins interacting with JEV NS3.
- Investigate the role of interleukin enhancer-binding factor 2 (ILF2) in JEV infection.
Main Methods:
- Co-immunoprecipitation assay
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
- Short hairpin RNA (shRNA) for gene knockdown
- JEV replicon system studies
Main Results:
- ILF2 was identified as a novel cellular protein interacting with JEV NS3.
- ILF2 expression decreased in JEV-infected cells.
- ILF2 knockdown enhanced JEV propagation and viral replication.
- ILF2 overexpression reduced JEV genome synthesis.
Conclusions:
- ILF2 negatively regulates JEV replication.
- ILF2 acts as a potential intrinsic antiviral factor against JEV infection.
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