Multiparametric Time-Correlated Single Photon Counting Luminescence Microscopy
V I Shcheslavskiy1,2, M V Shirmanova2, A Jelzow3
1Becker&Hickl GmbH, Berlin, 12277, Germany. vis@becker-hickl.de.
Biochemistry. Biokhimiia
|June 20, 2019
Summary
Time-correlated single photon counting (TCSPC) precisely measures photon arrival times for high-resolution imaging. Modern multi-dimensional TCSPC expands capabilities for advanced biological studies using fluorescence lifetime imaging.
Area of Science:
- Optics and Photonics
- Biophysics
- Biotechnology
Background:
- Classic time-correlated single photon counting (TCSPC) offers high time resolution and detection efficiency.
- Modern TCSPC integrates multiple data dimensions beyond photon arrival time.
Purpose of the Study:
- To review classic and multi-dimensional TCSPC microscopy techniques.
- To highlight applications in fluorescence lifetime imaging for biological research.
Main Methods:
- Detection of single photons from periodic optical signals.
- Registration of photon arrival times relative to reference pulses.
- Construction of multi-dimensional photon distributions.
Main Results:
- TCSPC achieves extremely high time resolution and near-ideal detection efficiency.
- Multi-dimensional TCSPC captures spatial, spectral, and temporal data for each photon.
- Enables advanced fluorescence lifetime imaging.
Conclusions:
- TCSPC is a powerful technique for precise timing in optical measurements.
- Multi-dimensional TCSPC significantly enhances fluorescence lifetime imaging capabilities.
- These advancements are crucial for diverse biological studies.
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