Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

11.7K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.7K
Regulated mRNA Transport02:22

Regulated mRNA Transport

7.0K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.0K
Regulated mRNA Transport02:22

Regulated mRNA Transport

3.3K
3.3K
pre-mRNA Processing02:01

pre-mRNA Processing

57.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.2K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.7K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

5.3K
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[MSCNet: Coronary artery segmentation network with multi-scale cascade encoding and dynamic spatial context enhancement].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi·2026
Same author

Mechanism Analysis of Fuzheng Xiaokui Formula in Treating Recurrent Aphthous Ulcers Based on Network Pharmacology.

Current pharmaceutical design·2026
Same author

Integrated multi-omics data and machine learning approaches to decipher the molecular network and gene signatures of renal cell carcinoma induced by aristolochic acid.

Biochemical and biophysical research communications·2026
Same author

Global burden and temporal trend of thyroid cancer associated with high BMI from 1990 to 2021.

PloS one·2026
Same author

Multi-omics reveals associations between the microbiota-gut-brain axis and antidepressant effects of vagus nerve stimulation.

Neurobiology of stress·2026
Same author

Twenty Natural Amino Acids Adsorption on Representative Surfaces: Dependence of Entropy-Enthalpy Compensation on Interface Properties.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Jan 23, 2026

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing
08:55

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

13.9K

An efficient method that combines the ThinPrep cytologic test with E6/E7 mRNA testing for cervical cancer screening.

Dan Pan1, Chan-Qiong Zhang1, Qi-Lian Liang2

  • 1Department of Pathology, the Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, Zhejiang, People's Republic of China.

Cancer Management and Research
|June 20, 2019
PubMed
Summary

Combined cervical cancer screening using ThinPrep cytologic test (TCT) and E6/E7 mRNA detection offers improved accuracy. This TCT+E6/E7 mRNA approach shows promise for widespread clinical use in cervical disease screening.

Keywords:
E6/E7 mRNAThinPrep cytologic testcervical cancerhuman papillomavirus DNA

More Related Videos

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection
08:09

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection

Published on: July 25, 2017

12.9K
Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.4K

Related Experiment Videos

Last Updated: Jan 23, 2026

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing
08:55

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

13.9K
Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection
08:09

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection

Published on: July 25, 2017

12.9K
Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.4K

Area of Science:

  • Oncology
  • Gynecologic Oncology
  • Molecular Diagnostics

Background:

  • Cervical cancer is linked to persistent human papillomavirus (HPV) infections.
  • Current screening methods include ThinPrep cytologic test (TCT), HPV DNA, and E6/E7 mRNA testing.
  • Accurate diagnosis is crucial, especially for atypical squamous cells of undetermined significance (ASCUS) results.

Purpose of the Study:

  • To identify an optimal diagnostic method for cervical cancer.
  • To evaluate screening methods for women with ASCUS or worse TCT results.
  • To compare the diagnostic performance of individual and combined screening techniques.

Main Methods:

  • Analysis of 301 patient samples from Wenzhou People's Hospital (June 2014-September 2017).
  • Comparative evaluation of TCT, HPV DNA detection, and E6/E7 mRNA testing.
  • Assessment of combined methods: TCT+HPV DNA, TCT+E6/E7 mRNA, and TCT+HPV DNA+E6/E7 mRNA.

Main Results:

  • The TCT+E6/E7 mRNA combination demonstrated superior diagnostic performance.
  • Sensitivity: 76.1% (95% CI: 0.659-0.841).
  • Specificity: 74.6% (95% CI: 0.681-0.803).
  • Highest Youden index of 0.507 for TCT+E6/E7 mRNA.

Conclusions:

  • Combined screening methods provide reliable specificity and sensitivity for cervical disease.
  • TCT+E6/E7 mRNA testing shows potential as a widely adopted clinical tool.
  • This approach can enhance cervical cancer screening accuracy.