LIN28B regulates androgen receptor in human trophoblast cells through Let-7c
Erin S McWhorter1, Rachel C West1, Jennifer E Russ1
1Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado.
Molecular Reproduction and Development
|June 20, 2019
Summary
LIN28B protein regulates androgen receptor (AR) levels in human trophoblast cells, likely via let-7c microRNA. This pathway may be crucial for trophoblast cell differentiation into syncytiotrophoblast.
Area of Science:
- Reproductive Biology
- Stem Cell Biology
- Molecular Endocrinology
Background:
- LIN28B is essential for stem cell pluripotency and trophoblast differentiation.
- LIN28B interacts with the Let-7 miRNA family to regulate gene expression.
- Androgen receptor (AR) regulation by LIN28B has been observed in cancer cells.
Purpose of the Study:
- To investigate the role of LIN28B in regulating androgen receptor (AR) levels in human trophoblast cells.
- To explore the potential involvement of the Let-7 miRNA family in this regulation.
Main Methods:
- Utilized the human first-trimester trophoblast cell line ACH-3P.
- Constructed a LIN28B knockdown cell line using lentiviral vectors.
- Administered Let-7c mimic and forskolin to evaluate effects on AR and syncytiotrophoblast markers.
Main Results:
- LIN28B knockdown led to decreased AR levels and increased Let-7 miRNAs.
- Let-7c mimic treatment reduced both LIN28B and AR levels.
- Forskolin-induced syncytialization and Let-7c treatment increased ERVW-1 expression and decreased AR levels.
Conclusions:
- LIN28B regulates AR levels in trophoblast cells, potentially through inhibition of let-7c.
- This LIN28B-let-7c-AR axis may be vital for human trophoblast cell differentiation into syncytiotrophoblast.
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