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Gonadotropin measurements with fast, non-radioactive methods
12nd Dep. of Gynecology and Obstetrics, University of Vienna.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|October 1, 1987
Summary
Comparing analytical methods for lutropin and follitropin measurement, this study found high correlation between DELFIA and radioimmunoassay for serum samples. Immunologic assays offer a viable alternative to traditional methods.
Area of Science:
- Reproductive endocrinology
- Biochemical analysis
- Immunoassay development
Background:
- Accurate measurement of lutropin (LH) and follitropin (FSH) is crucial for reproductive health assessment.
- Various analytical methods exist, but their comparative performance requires evaluation.
Purpose of the Study:
- To compare different analytical methods for measuring lutropin and follitropin.
- To assess the reliability and correlation of various immunoassay techniques.
Main Methods:
- Lutropin in urine was measured using HI Gonavis (semiquantitative), Ovustick (quantitative), and DELFIA.
- Serum lutropin and follitropin levels were analyzed using DELFIA and radioimmunoassay (RIA).
Main Results:
- Semiquantitative and quantitative urine lutropin assays showed conformity in 37 out of 40 menstrual cycles.
- Serum DELFIA and RIA demonstrated high correlation for both lutropin (r=0.970) and follitropin (r=0.994).
Conclusions:
- Immunologic assays utilizing enzyme- or europium-labelled tracers are practical alternatives to haemagglutination or RIA.
- DELFIA offers a reliable and highly correlated method for serum LH and FSH quantification.