Related Experiment Video

Updated: Jan 23, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

24.1K

Anticancer effect of inactivated Sendai virus strain Tianjin on human osteosarcoma HOS cells

Qing Li1, Huachong Ma2, Shuya Sun

  • 1Department of Pathogenic Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. liqing2@tmu.edu.cn.

General Physiology and Biophysics
|June 21, 2019
PubMed

Insights

Ultraviolet-inactivated Sendai virus strain Tianjin (UV-Tianjin) effectively induces apoptosis in human osteosarcoma cells. This antitumor effect involves mitochondrial dysfunction, reactive oxygen species generation, and the MAPK pathway.

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • Ultraviolet-inactivated Sendai virus strain Tianjin (UV-Tianjin) exhibits known antitumor properties.
  • Osteosarcoma remains a significant challenge in orthopedic oncology.

Purpose of the Study:

  • To investigate the anticancer effects of UV-Tianjin on human osteosarcoma HOS cells.
  • To elucidate the molecular mechanisms underlying UV-Tianjin's antitumor activity.

Main Methods:

  • Flow cytometry was used to assess apoptosis, intracellular reactive oxygen species (ROS) levels, and mitochondrial membrane potential.
  • Western blotting analyzed the expression of apoptosis-related proteins.
  • Mitogen-activated protein kinase (MAPK) inhibitors and ROS inhibitors were employed.

Main Results:

  • UV-Tianjin induced apoptosis in HOS cells in a dose-dependent manner.
  • Apoptosis was mediated by the mitochondrial pathway, evidenced by mitochondrial dysfunction and altered expression of Bcl-2 family proteins (Bcl-2, Bcl-xL, Mcl-1, Bax, Bak).
  • UV-Tianjin treatment increased ROS generation and activated MAPK pathways (JNK, ERK, p38), which were crucial for apoptosis induction.

Conclusions:

  • UV-Tianjin exerts antitumor effects on human osteosarcoma cells by triggering mitochondria-dependent apoptosis.
  • The mechanism involves ROS generation and activation of the MAPK signaling pathway.

More Related Videos

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions09:32

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Published on: November 11, 2015

12.5K
Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation09:57

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Published on: December 21, 2016

15.2K

Related Concept Videos

X-Inactivation01:58

X-Inactivation

The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
41.7K
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
11.3K
What are Viruses?00:50

What are Viruses?

Overview
127.9K
Thermal Strain01:19

Thermal Strain

Thermal strain is a concept that arises when we consider how temperature changes affect structures. Unlike the conventional assumption that structures remain constant under load, real-world scenarios often involve temperature fluctuations that can significantly impact these structures. Consider a homogeneous rod with a uniform cross-section resting freely on a flat horizontal surface. If the rod's temperature increases, the rod elongates. This elongation is proportional to the temperature...
2.8K
Shearing Strain01:20

Shearing Strain

The shearing strain represents a cubic element's angular change when subjected to shearing stress. This type of stress can transform a cube into an oblique parallelepiped without influencing normal strains. The cubic element experiences a significant transformation when exposed solely to shearing stress. Its shape alters from a perfect cube into a rhomboid, clearly demonstrating the effect of shearing strain. The degree of this strain is considered positive if it reduces the angle between the...
1.3K
Measurements of Strain01:27

Measurements of Strain

Strain quantifies the deformation of a material under force, typically measured as normal strain, which represents the change in length when compared with the original length. Electrical strain gauges are used for enhanced accuracy. These devices consist of a conductive wire mounted on a paper backing that adheres to the material's surface. These gauges operate on the piezoresistive effect, where the wire's electrical resistance changes in response to mechanical deformation. The strain...
2.6K