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Passive filters are utilized to shape the frequency spectrum of signals across a diverse array of applications. These filters, using only passive elements like resistors (R), inductors (L), and capacitors (C), are capable of selectively allowing or blocking certain frequency ranges without the need for external power sources.
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Related Experiment Video

Updated: Jan 23, 2026

Air-sampled Filter Analysis for Endotoxins and DNA Content

Air-sampled Filter Analysis for Endotoxins and DNA Content

Published on: March 7, 2016

DNA RETENTION ON DEPTH FILTERS.

Ohnmar Khanal¹, Xuankuo Xu², Nripen Singh²

¹Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.

Journal of Membrane Science

|June 22, 2019

Summary

This summary is machine-generated.

Understanding how depth filters retain impurities like double-stranded DNA (dsDNA) is crucial for bioprocessing. This study reveals DNA retention mechanisms, showing smaller DNA fragments are retained more effectively within depth filter media.

Keywords:

DNA oligos adsorption depth filtration genomic DNA retention

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Last Updated: Jan 23, 2026

Air-sampled Filter Analysis for Endotoxins and DNA Content

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Published on: March 7, 2016

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DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation

Published on: September 18, 2009

Area of Science:

Bioprocessing
Filtration technology
Molecular biology

Background:

Depth filtration is vital for removing impurities during bioprocessing.
Understanding impurity retention mechanisms in depth filters is complex due to filter composition.
Double-stranded DNA (dsDNA) is a key impurity that needs efficient removal.

Purpose of the Study:

To investigate the mechanisms of dsDNA retention within depth filter media.
To elucidate the influence of DNA size and operational parameters on retention.
To visualize DNA distribution within spent depth filter discs.

Main Methods:

Confocal fluorescence microscopy was used to visualize fluorescently-labeled dsDNA distribution.
Experiments varied DNA length, wash volume, and buffer ionic strength.
Cellular DNA was labeled using 5-ethynyl-2'-deoxyuridine (EdU) in Chinese Hamster Ovary (CHO) cells.

Main Results:

DNA displacement into the depth filter increased with decreasing DNA length.
Increased wash volume and buffer ionic strength enhanced DNA displacement.
CHO cellular DNA migrated deeper into the filter than pelletized DNA, highlighting size-dependent migration.

Conclusions:

DNA size significantly impacts its migration and retention within depth filters.
Operational parameters like wash volume and ionic strength modulate DNA retention.
The study provides insights into nucleic acid and impurity removal in bioprocessing, with potential for small-scale studies.