Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synthetic Surrogates of Collagen-Rich Microenvironments: Integrating Modular Bioactive Fibrillar Structure and Tunable Viscoelasticity via Multifunctional Assembling Peptides.

ACS central science·2026

Same author

Light-activated thiomaleimide crosslinking stabilizes collagen mimetic peptide triple helix.

Chemical communications (Cambridge, England)·2026

Same author

<i>Legionella pneumophila</i> Encodes a Peptidoglycan Recycling Machinery Critical for Survival within Macrophages.

ACS infectious diseases·2026

Same author

Role of viral protein ratio in the structure and separation of empty and full adeno-associated virus capsids: A molecular dynamics study.

Molecular therapy. Advances·2026

Same author

Matrix stiffening toolbox: dynamic hydrogels for three-dimensional cell culture with real-time cell response.

bioRxiv : the preprint server for biology·2026

Same author

Enhancing Membrane Adhesion to Polymeric Substrates via Plasma Treatment.

ACS applied polymer materials·2026

Same journal

Research advances and application prospects of CAR-T therapy in the treatment of age-related diseases.

Frontiers in immunology·2026

Same journal

Machine learning-driven identification and immunohistochemical validation of an integrated immune-inflammatory phenotype for disease-free survival stratification in breast cancer.

Frontiers in immunology·2026

Same journal

Modified treatment protocol for pediatric systemic lupus erythematosus-associated hemophagocytic lymphohistiocytosis with central nervous system involvement: a case report.

Frontiers in immunology·2026

Same journal

Exploratory characterization of IgG1/IgG4 glycosylation and monocyte-derived dendritic cell responses in esophageal squamous cell carcinoma.

Frontiers in immunology·2026

Same journal

JAK-STAT pathway-associated skin diseases: a refined functional framework for inflammatory skin diseases.

Frontiers in immunology·2026

Same journal

Cross-talk among novel programmed cell death pathways: a decisive network in renal ischemia-reperfusion injury.

Frontiers in immunology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 15, 2025

Manufacturing Chimeric Antigen Receptor CAR T Cells for Adoptive Immunotherapy

Manufacturing Chimeric Antigen Receptor CAR T Cells for Adoptive Immunotherapy

Published on: December 17, 2019

Bio-functional hydrogel coated membranes to decrease T-cell exhaustion in manufacturing of CAR T-cells.

Aida López Ruiz¹, Eric Slaughter¹, Kartik Bomb¹

¹Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, United States.

Frontiers in Immunology

|July 14, 2025

Summary

This summary is machine-generated.

Bioinspired hydrogel-coated membranes improve chimeric antigen receptor (CAR) T-cell production by reducing T-cell exhaustion. This leads to enhanced CAR T-cell quality and more effective cancer treatment.

Keywords:

CAR T T cell bioinspired biomaterials cell therapy coating hydrogel ligand

More Related Videos

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Published on: December 23, 2022

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Published on: September 9, 2022

Related Experiment Videos

Last Updated: Sep 15, 2025

Manufacturing Chimeric Antigen Receptor CAR T Cells for Adoptive Immunotherapy

Manufacturing Chimeric Antigen Receptor CAR T Cells for Adoptive Immunotherapy

Published on: December 17, 2019

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Published on: December 23, 2022

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Published on: September 9, 2022

Area of Science:

Biotechnology
Immunology
Materials Science

Background:

Chimeric antigen receptor (CAR) T-cell therapies are vital for treating hematological cancers.
Current CAR T-cell manufacturing can lead to T-cell exhaustion, reducing treatment efficacy.
Controlling T-cell activation is crucial for improving CAR T-cell quality.

Purpose of the Study:

To develop a bioinspired, scalable platform to control T-cell activation and reduce exhaustion during CAR T production.
To investigate the impact of hydrogel-coated membranes (HCMs) on T-cell phenotype, activation, and exhaustion.
To evaluate the efficacy of CAR T-cells produced using HCMs.

Main Methods:

Designed HCMs with co-stimulatory ligands and a physiologically relevant substrate modulus.
Compared T-cells cultured on HCMs versus industry-standard TransAct, assessing phenotype, activation, and exhaustion markers.
Transduced T-cells with a CD19 CAR lentivirus and evaluated in vitro cytolysis.

Main Results:

HCMs promoted memory T-cell phenotypes with minimal exhaustion and comparable activation.
CAR T-cell production using HCMs resulted in increased transduction efficiency and reduced exhaustion.
CAR T-cells generated with HCMs demonstrated improved in vitro cytolysis of target cells with reduced variability.

Conclusions:

Controlled T-cell activation using bioinspired materials like HCMs is critical for enhancing CAR T-cell manufacturing.
Reduced T-cell exhaustion is key to improving the quality and efficacy of CAR T-cell therapies.
Engineered bioinspired materials offer significant potential for modulating T-cell phenotypes in cell therapy applications.