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Dram1 regulates DNA damage-induced alternative autophagy
Meruna Nagata1, Satoko Arakawa1, Hirofumi Yamaguchi1,2
1Department of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Cell Stress
|June 22, 2019
Summary
Genotoxic stress induces alternative autophagy via p53 and damage-regulated autophagy modulator (Dram1). Dram1 alone can trigger this process, highlighting its crucial role in cellular repair mechanisms.
Area of Science:
- Cellular Biology
- Molecular Mechanisms
- Autophagy Research
Background:
- Autophagy is a conserved cellular degradation process.
- Mammalian cells exhibit Atg5-dependent and Atg5-independent autophagy.
- Key molecules for alternative autophagy remain largely unidentified.
Purpose of the Study:
- To investigate the molecular mechanisms of genotoxic stress-induced alternative autophagy.
- To identify novel proteins involved in Atg5-independent autophagy.
- To elucidate the role of p53 and Dram1 in this pathway.
Main Methods:
- Analysis of molecular mechanisms underlying genotoxic stress.
- Identification of essential proteins through experimental assays.
- Functional studies of p53 and Dram1 in autophagy induction.
Main Results:
- p53 and damage-regulated autophagy modulator (Dram1) were identified as essential for alternative autophagy.
- Dram1 was found to be sufficient for inducing alternative autophagy.
- Dram1 acts downstream of p53 in regulating isolation membrane closure during autophagy.
Conclusions:
- Dram1 plays a pivotal role in genotoxic stress-induced alternative autophagy.
- The p53-Dram1 axis is critical for Atg5-independent autophagy.
- This study advances understanding of alternative autophagy pathways.
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