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Too Much Compromise in Today's CRISPR Pipelines.
1Data Science, Inscripta, Inc., Boulder, Colorado.
The CRISPR Journal
|June 22, 2019
Summary
CRISPR gene editing advances genome engineering but requires more scalable methods. Current limitations restrict the types of edits possible, hindering broader research applications and the technology's full potential.
Area of Science:
- Genetics and Genomics
- Molecular Biology
- Biotechnology
Background:
- CRISPR-based gene editing is a transformative technology for biological research.
- Rapid advancements have occurred, yet capabilities remain limited.
- Current scalable editing methods are restricted to specific edit types.
Purpose of the Study:
- To highlight the limitations in current CRISPR editing technologies.
- To identify the need for expanded capabilities in scalable gene editing.
- To underscore the importance of further advancements for realizing CRISPR's full potential.
Main Methods:
- This study reviews the current state of CRISPR-based editing technologies.
- It analyzes the limitations in scalability (massively parallel and multiplex) editing.
- The review focuses on the variety of edit types currently supported.
Main Results:
- Scalable CRISPR editing is currently limited to a narrow range of edit types.
- This limitation restricts the scope and types of research studies possible.
- Significant gaps exist in the available CRISPR editing toolkit.
Conclusions:
- Further innovation is essential to overcome current limitations in CRISPR gene editing.
- Expanding the repertoire of scalable editing types is crucial for advancing biological discovery.
- Realizing the full potential of CRISPR technology necessitates continued development and refinement.