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Updated: Jan 23, 2026
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Using Primary Neurosphere Cultures to Study Primary Cilia
Published on: April 14, 2017
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Primary cilia in murine palatal rugae development.
Mayuko Nakaniwa1, Maiko Kawasaki1, Katsushige Kawasaki2
1Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Gene Expression Patterns : GEP
|June 22, 2019
Summary
Primary cilia are crucial for forming the periodic patterns of mammalian palatal rugae. Deleting the primary cilia protein Ift88 disrupted rugae development and altered key signaling pathways.
Area of Science:
- Developmental Biology
- Cell Biology
- Genetics
Background:
- Periodic patterning of iterative structures is vital in embryonic development and organogenesis.
- Palatal rugae, the ridges on the hard palate, serve as a model for studying periodic patterning.
- The role of primary cilia in palatal rugae development is not well understood.
Purpose of the Study:
- To investigate the function of primary cilia in the development of palatal rugae.
- To elucidate the molecular mechanisms underlying palatal rugae formation, focusing on cilia-dependent signaling pathways.
Main Methods:
- Localization analysis of primary cilia in developing palatal rugae.
- Generation of mice with conditional deletion of the primary cilia protein Ift88 in placodal ectoderm (Ift88fl/fl;ShhCre).
- Comparative in situ hybridization to analyze expression patterns of Shh and Wnt signaling molecules.
Main Results:
- Primary cilia were specifically localized to the interplacode epithelium of palatal rugae.
- Conditional deletion of Ift88 resulted in highly disorganized palatal rugae.
- Expression of Shh and non-canonical Wnt signaling molecules was altered in Ift88-deficient mice.
Conclusions:
- Primary cilia play a significant role in the development of palatal rugae.
- Cilia-mediated regulation of Shh and Wnt signaling pathways is essential for proper palatal rugae patterning.
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