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Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
The complete genome sequence of Bifidobacterium longum LTBL16, a potential probiotic strain from healthy centenarians
Guohong Huang1, Haibo Pan2, Zhenjun Zhu2
1College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, People's Republic of China; College of Food and Biological Technology, Guangxi Vocational and Technical College, Ming Yang Industrial Park Jiangnan District, Nanning 530226, People's Republic of China.
Abstract:
B. longum LTBL16 is a potential probiotic strain that was isolated from healthy centenarians in Bama, China. In vitro experiments show that B. longum LTBL16 has a strong antioxidant activity and the complete genome of B. longum LTBL16 was sequenced in this work. The genome consists of one 2,430,682 bp circular chromosome that is plasmid free. The circular chromosome has a GC content of 61.23% and contains 2071 coding sequences (CDSs), 4 rRNA manipulators and 55 tRNA coding genes. Genetic analysis showed that at least five protein-coding genes were associated with antioxidant activity, and the abundance of these genes may be related to free radical scavenging rates and oxygen tolerance. In addition, the safety of B. longum LTBL16 was evaluated using a virulence factor database and antibiotic resistance gene database. The results indicate that B. longum LTBL16 has the good potential for the development and utilization as a probiotic.
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