Related Experiment Video
Updated: Jan 23, 2026

Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field
Published on: August 27, 2021
Kaempferol attenuates mitochondrial dysfunction and oxidative stress induced by H2O2 during porcine embryonic
Xuerui Yao1, Hao Jiang2, Yong NanXu3
1College of Agriculture, Yanbian University, Yanji, 133000, China; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea.
Abstract:
Kaempferol (3,4',5,7-tetrahydroxyflavone, KAE) is one of the most commonly occurring dietary flavonols. The biological and pharmacological effects of kaempferol depend upon whether it acts as an antioxidant, anti-inflammatory, or anticancer agent. The present study explored the influence of KAE supplementation on in vitro damage to porcine oocytes and its underlying mechanisms. Different concentrations of KAE (0.05, 0.1, 0.5, 1 μM) were added to porcine zygote medium 5 during in vitro culture. The results showed that supplementation with 0.1 μM KAE significantly increased the blastocyst formation rate. Blastocyst formation and quality were significantly increased in the 200 μM H2O2 treatment group following addition of 0.1 μM KAE. KAE prevented the H2O2-induced compromise of mitochondrial membrane potential and reactive oxygen species generation. Furthermore, the extent of autophagy and DNA damage in the blastocysts was attenuated by supplementation with KAE in the H2O2 induced oxidative injury group compared to that observed in controls. These results suggest that KAE has beneficial effects on the development of porcine parthenotes by attenuating oxidative stress and increasing mitochondrial function.
Related Concept Videos
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Animal Mitochondrial Genetics
Oxidation Numbers
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Export of Mitochondrial and Chloroplast Genes
Pyruvate Oxidation
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...

