Related Experiment Video

Updated: Jan 23, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

MiR-130a exerts neuroprotective effects against ischemic stroke through PTEN/PI3K/AKT pathway

Tingting Zheng¹, Yu Shi¹, Jun Zhang²

¹Shenzhen Key Laboratory for Drug Addiction and Medication Safety, Department of Ultrasound, Peking University Shenzhen Hospital, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen 518036, Guangdong Province, PR China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie

|June 22, 2019

Abstract

Background:

Ischemic stroke is significantly affected by the dysfunction of the miRNA network. Recent research has described that disordered expression of miR-130a is associated with ischemic stroke. Here, we aimed to investigate the possible mechanism of the miR-130a-mediated neuroprotection that follows ischemia-reperfusion (I/R) injury.

Method:

This study was comprised of two models: oxygen-glucose deprivation/Reperfusion (OGDR) and middle cerebral artery occlusion (MCAO). RT-PCR and immunoblotting were used to examine gene expression levels, and MTT assay and flow cytometric analysis were used to examine cell states. We also used 2, 3, 5-triphenyltetrazolium chloride (TTC) staining to assess the cerebral infarct volume. Then, we employed bioinformatics analysis and luciferase reporter assay to identify and validate the target molecule of miR-130a, PTEN.

Results:

Our findings indicated that miR-130a expression was lower in PC12 cells after OGDR (oxygen-glucose deprivation/reperfusion) and in rats after MCAO (middle cerebral artery occlusion). Moreover, ectopic-expression of miR-130a can significantly improve cell survival rate and reduce cell apoptosis and ROS production in PC12 cells after OGDR. In addition, re-expression of miR-130a yielded an obvious reduction in MCAO-induced infarct volume and neurological deficits in rats. Bioinformatics analysis revealed that PTEN was a miR-130a target and could overturn the effect of miR-130a on cerebral ischemia, both in vivo and in vitro. Therefore, we set out to further investigate the PTEN-affected PI3K/AKT pathway and found that upregulation of miR-130a activated the PI3K/AKT pathway.

Conclusions:

Our data demonstrated that miR-130a prevented cerebral I/R damage by mediating the PTEN/PI3K/AKT axis. These preliminarily findings furthered our understanding of this mechanism and identified new potential therapeutic targets for ischemic stroke.

Insights

MicroRNA-130a (miR-130a) protects the brain from ischemia-reperfusion injury by targeting PTEN and activating the PI3K/AKT pathway. This finding offers a potential therapeutic strategy for ischemic stroke.

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Dysfunctional miRNA networks significantly impact ischemic stroke outcomes.
Reduced miR-130a expression is linked to ischemic stroke development.
Investigating miR-130a's neuroprotective mechanisms in ischemia-reperfusion (I/R) injury is crucial.

Purpose of the Study:

To elucidate the mechanism of miR-130a-mediated neuroprotection against I/R injury.
To identify the molecular targets of miR-130a in the context of cerebral ischemia.
To explore the therapeutic potential of modulating miR-130a in ischemic stroke.

Main Methods:

Utilized oxygen-glucose deprivation/reperfusion (OGDR) and middle cerebral artery occlusion (MCAO) models.
Assessed gene and protein expression via RT-PCR and immunoblotting.
Evaluated cell viability, apoptosis, ROS production, infarct volume, and neurological deficits.
Employed bioinformatics, luciferase reporter assays, and pathway analysis (PTEN/PI3K/AKT).

Main Results:

miR-130a expression was downregulated in both in vitro (OGDR) and in vivo (MCAO) models of cerebral ischemia.
Overexpression of miR-130a enhanced cell survival, reduced apoptosis and ROS production, decreased infarct volume, and improved neurological function.
PTEN was identified as a direct target of miR-130a, and its inhibition reversed miR-130a's protective effects.
Upregulation of miR-130a activated the PI3K/AKT pathway by inhibiting PTEN.

Conclusions:

miR-130a confers neuroprotection against cerebral I/R injury by targeting PTEN and modulating the PTEN/PI3K/AKT signaling axis.
These findings enhance the understanding of molecular mechanisms underlying ischemic stroke.
miR-130a represents a promising therapeutic target for managing ischemic stroke.

Read full analysis

Keywords:

Cerebral ischemia-reperfusion PI3K/AKT pathway PTEN miR-130a

More Related Videos

A Preclinical Model of Exertional Heat Stroke in Mice

A Preclinical Model of Exertional Heat Stroke in Mice

Published on: July 1, 2021

Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents

Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents

Published on: June 2, 2015

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

C4 Pathway and CAM

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...

Other Glycolytic Pathways

Other Glycolytic Pathways

The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...

Respiration Pathways

Respiration Pathways

Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...

Auditory Pathway

Auditory Pathway

Auditory pathways constitute the complex neural circuits responsible for transmitting and interpreting auditory information from the peripheral auditory system to the brain. Sound waves are initially captured by the outer ear, funneled through the ear canal, and reach the tympanic membrane (eardrum). These vibrations are transmitted via the middle ear's ossicles to the inner ear's cochlea.
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking...

ER Retrieval Pathway

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...

Articles linked to this work by shared authors, journal, and citation graph.

A New Constitutive Model for Shale under Hydro-Mechanical Coupling Based on the Disturbed State Concept Theory.

ACS omega·2026

A Rare Cardiac Triple Siege in Youth: Right Atrial Lipoma, Coronary Stenosis, and Myocardial Bridge.

Journal of clinical ultrasound : JCU·2026

Toxoplasma gondii hijacks host S100A6 to enhance cell migration and brain cyst formation.

Communications biology·2026

Study on the effect of substrate temperature on the infrared transmittance of DLC films fabricated by magnetron sputtering.

Optics express·2026

Pharmacokinetic Drug-Drug Interaction of Leritrelvir in Healthy Chinese Volunteers: A Single-Arm, Open-Label, Dual-Cohort Study.

Clinical drug investigation·2026

CCR5 as a key mediator: Insights into the molecular mechanisms by which air pollutants induce Alzheimer's disease via network toxicology and molecular docking.

Brain research bulletin·2026

See all related articles