Co-pelletization of microalgae and fungi for efficient nutrient purification and biogas upgrading

The fungi-assisted microalgae pellets were cultivated to simultaneously purify biogas slurry and biogas. Results demonstrated that the optimized culture conditions for the pellets were at the shaking speed of 160 rpm, initial inoculum concentration of 1.0 × 10⁶ spores L^-1 for microalgal cells, fungi/algae ratio of 1:10, mixed medium/simulated biogas slurry ratio of 3:7 incubated at a light intensity of 200 μmol m^-2 s^-1. Moreover, biogas slurry purification and biogas upgrading were successfully achieved by co-cultivation of fungi-assisted microalgae pellets at mixed light wavelength of red:blue of 5:5. The highest removal efficiency of COD, TN, TP and CO₂ were 92.17 ± 5.28%, 89.83 ± 4.36%, 90.31 ± 4.69% and 74.26 ± 3.14%, respectively. These results will provide a theoretical foundation for large-scale biogas slurry purification and biogas upgrading using fungi-assisted microalgal pellets.