Related Experiment Video
Updated: Jan 23, 2026
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
Published on: February 25, 2007
14.0K
Embryonic Stem Cells and Gene Manipulation in Rat
Masumi Hirabayashi1, Akiko Takizawa2, Shinichi Hochi3
1Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, Japan. mhirarin@nips.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2019
Summary
Generating gene-modified rats using embryonic stem cells (ESCs) is crucial for neuroscience and pharmacology research. This chapter details protocols for establishing rat ESCs and producing genetically altered rats for advanced biological studies.
Area of Science:
- Biotechnology and Genetic Engineering
- Neuroscience Research Tools
- Pharmacology and Toxicology Models
Background:
- Transgenic rat technology is vital for biological and physiological studies.
- Embryonic stem cells (ESCs) are key to generating gene-modified rats.
- Rat ESCs now allow both foreign DNA introduction and endogenous DNA modification.
Purpose of the Study:
- To provide detailed protocols for establishing authentic rat ESCs.
- To outline methods for using rat ESCs to produce gene-modified rats.
- To support advancements in neuroscience, pharmacology, and toxicology research.
Main Methods:
- Establishment of pluripotent rat ESC lines.
- Genetic modification of rat ESCs (transgenesis and gene editing).
- Production of gene-modified rats via ESCs.
Main Results:
- Successful establishment of bona fide rat ESCs.
- Demonstrated efficacy of rat ESCs for gene modification.
- Generation of gene-modified rats using established protocols.
Conclusions:
- Detailed protocols enable reliable generation of gene-modified rats.
- Rat ESCs are a powerful platform for creating sophisticated research models.
- This work facilitates future studies in neuroscience, pharmacology, and toxicology.
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