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Updated: Jan 23, 2026

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
Published on: March 17, 2023
Design of a Nile red-based NIR fluorescent probe for the detection of hydrogen peroxide in living cells
Quanping Diao1, Hua Guo1, Zhiwei Yang1
1School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan 114005, China.
Abstract:
In this article, a novel fluorescent probe (NRBE) for detecting H2O2 was developed using benzyl boronic ester as the H2O2-recognized group and Nile red as the matrix. The probe has several advantages, such as good selectivity, high sensitivity (LOD = 75 nM), good water solubility and emission in the near-infrared region (ex/em:585/670 nm). With the NRBE probe, the endogenous H2O2 in human hepatocellular carcinoma cells BEL-7402, was detected, and the H2O2 generated during the ischemia-reperfusion of the cells was imaged. These results show that NRBE can be applied for real-time detection of H2O2 in biological systems.
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