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Updated: Jan 23, 2026

Gene Editing of Primary Rhesus Macaque B Cells
Published on: February 10, 2023
In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos
1Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA.
Abstract:
Production of nonhuman primate (NHP) embryos in vitro begins with recovery of gametes. Females undergo a controlled ovary stimulation to produce multiple preovulatory follicles from which oocytes may be recovered. Mature ova are subjected to in vitro fertilization (IVF) and presumptive zygotes are cultured to the intended stage of development. Essential to this practice is the culture medium unique to each step in the process. Here we describe medium preparation, oocyte recovery, in vitro fertilization, and in vitro culture (IVC) of embryos in the rhesus macaque model.
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