Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Frustration and Conflict: Approach-Approach, Approach-Avoidance01:20

Frustration and Conflict: Approach-Approach, Approach-Avoidance

497
Frustration occurs when people are obstructed or prevented from achieving a desired goal or fulfilling a perceived need. For example, when someone's input is ignored in a discussion, it can lead to feelings of frustration. Conflict, however, arises from opposing interests, goals, or actions. Conflicts can take various forms based on the nature of these opposing desires or goals.
One common type of conflict is the Approach–Approach Conflict. In this case, a person faces two desirable...
497
Information Processing Approach01:30

Information Processing Approach

538
The information-processing theory of cognitive development centers on fundamental mental processes, including attention, memory, and problem-solving skills. Researchers in this field examine how cognitive abilities, such as working memory, evolve and influence children's overall development. Studies indicate that children with stronger working memory tend to excel in reading comprehension, math, and problem-solving compared to peers with less efficient memory skills. Low working memory is...
538
Actuarial Approach01:20

Actuarial Approach

292
The actuarial approach, a statistical method originally developed for life insurance risk assessment, is widely used to calculate survival rates in clinical and population studies. This method accounts for participants lost to follow-up or those who die from causes unrelated to the study, ensuring a more accurate representation of survival probabilities.
Consider the example of a high-risk surgical procedure with significant early-stage mortality. A two-year clinical study is conducted,...
292
The Nativist Approach01:21

The Nativist Approach

407
The nativist approach to infant cognitive development proposes that infants are born with inherent knowledge structures that allow them to interpret the world almost immediately. This perspective contrasts with earlier developmental theories, such as those proposed by Jean Piaget, which emphasized a more gradual acquisition of cognitive abilities through interaction with the environment. One key concept in this approach is object permanence — the understanding that objects continue to...
407
Cancer02:18

Cancer

53.7K
Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.
53.7K
What is Cancer?02:12

What is Cancer?

14.0K
Cells and tissues must meticulously coordinate their activities for the normal functioning of the human body. Therefore, they exhibit socially responsible behavior - resting, growing, dividing, differentiating, or dying - for the organism’s benefit. Cancer arises when cells divide uncontrollably and invade other tissues or organs.
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of...
14.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Personalized Risk Stratification of Residual Histologic Activity in IBD Using Circulating Cytokines.

Journal of personalized medicine·2026
Same author

Microbial collagenase activity is linked to oral-gut translocation in advanced chronic liver disease.

Nature microbiology·2025
Same author

Plasmin-mediated proteolysis of von Willebrand factor in patients with acute and chronic liver disease.

Research and practice in thrombosis and haemostasis·2025
Same author

The Level of Adherence to Organic Food Consumption and Risk of Cancer: A Systematic Review and Meta-Analysis.

Life (Basel, Switzerland)·2025
Same author

Oral-gut microbiome interactions in advanced cirrhosis: characterisation of pathogenic enterotypes and salivatypes, virulence factors and antimicrobial resistance.

Journal of hepatology·2024
Same author

microRNA associated with hepatocyte injury and systemic inflammation may predict adverse outcomes in cirrhotic patients.

Scientific reports·2024

Related Experiment Video

Updated: Jan 23, 2026

IP-FCM: Immunoprecipitation Detected by Flow Cytometry
12:17

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

Published on: December 2, 2010

17.9K

Colorectal Cancer CTCs Detection Using Two FCM Protocol Approaches.

Antonia Mourtzikou, Marilena Stamouli, Petros Karakitsos

    Clinical Laboratory
    |June 25, 2019
    PubMed
    Summary

    This study developed two low-cost flow cytometry methods to detect circulating tumor cells (CTCs) in colorectal cancer patients. These methods offer a more accessible approach for identifying CTCs, aiding in personalized cancer therapy.

    More Related Videos

    Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection
    07:35

    Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

    Published on: June 8, 2020

    7.3K
    Orthotopic Mouse Model of Colorectal Cancer
    08:43

    Orthotopic Mouse Model of Colorectal Cancer

    Published on: December 4, 2007

    47.1K

    Related Experiment Videos

    Last Updated: Jan 23, 2026

    IP-FCM: Immunoprecipitation Detected by Flow Cytometry
    12:17

    IP-FCM: Immunoprecipitation Detected by Flow Cytometry

    Published on: December 2, 2010

    17.9K
    Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection
    07:35

    Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

    Published on: June 8, 2020

    7.3K
    Orthotopic Mouse Model of Colorectal Cancer
    08:43

    Orthotopic Mouse Model of Colorectal Cancer

    Published on: December 4, 2007

    47.1K

    Area of Science:

    • Oncology
    • Biotechnology
    • Medical Diagnostics

    Background:

    • Circulating tumor cells (CTCs) are crucial biomarkers for personalized cancer therapy and therapeutic target discovery.
    • Current CTC identification methods like CellSearch® and Amnis® are effective but can be complex and costly.
    • Developing accessible methods for CTC detection is essential for broader clinical application.

    Purpose of the Study:

    • To establish and evaluate two cost-effective flow cytometry (FCM) protocols for identifying and enumerating CTCs in colorectal cancer (CRC) patients.
    • To compare the efficacy of these novel FCM protocols against existing methods.
    • To assess the correlation of CTC counts with clinical parameters in CRC.

    Main Methods:

    • Two distinct FCM protocols (direct and intracellular) were developed and validated using immunomagnetic enrichment.
    • CTC enumeration was performed on blood samples from CRC patients before and after surgery.
    • Blood from healthy donors served as a negative control, and the Cyflow® Space cytometer was utilized.

    Main Results:

    • Both FCM protocols successfully detected and enumerated CTCs in CRC patients, showing a statistically significant increase in CTC numbers within the patient group (p < 0.05).
    • The intracellular protocol demonstrated higher sensitivity (62.7%) and specificity (70%) compared to the direct protocol (49.2% SN, 58.3% SP).
    • Significant correlations were observed between intracellular CTC counts and adenocarcinoma, lymph node infiltration, and disease stage (p < 0.05).

    Conclusions:

    • Two novel, low-cost FCM protocols for CTC detection in colorectal cancer were successfully established.
    • These methods provide a viable alternative for CTC enumeration, potentially improving accessibility in clinical settings.
    • While CTC numbers increased significantly in patients, no significant difference was found before and after surgery, warranting further investigation.