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The ABO Blood Group01:12

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Related Experiment Video

Updated: Jan 23, 2026

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
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Method for ABO Blood Group Testing Using a General-Purpose Automated Biochemical Analyzer.

Satoshi Tada, Shoichi Kanayama, Akemi Miyagawa

    Clinical Laboratory
    |June 25, 2019
    PubMed
    Summary

    A new method for ABO blood typing uses a standard biochemical analyzer for direct red blood cell sampling, offering high precision and speed. This validated approach achieved 100% agreement with traditional methods in large-scale patient testing.

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    Area of Science:

    • Clinical Chemistry
    • Hematology
    • Medical Diagnostics

    Background:

    • Traditional ABO blood typing methods can be time-consuming.
    • There is a need for efficient and accurate blood group determination.

    Purpose of the Study:

    • To develop and validate a high-throughput, high-precision forward ABO blood typing method.
    • To utilize a general-purpose biochemical analyzer for direct red blood cell sampling.

    Main Methods:

    • Employed Ortho® BioClone® Anti-A and Anti-B Sera with AFFIRMAGEN® Reagent Red Blood Cells.
    • Utilized a TBA™-120FR HbA1c analyzer for automated sample processing.
    • Determined ABO blood group based on AFFIRMAGEN® response changes.

    Main Results:

    • Achieved repeatability with a coefficient of variation (CV) of 5% or lower.
    • Demonstrated 100% agreement with the tube test method in 1,112 patient samples.
    • Validated the accuracy and reliability of the automated method.

    Conclusions:

    • The developed method provides a simple, rapid, and precise approach to ABO blood typing.
    • This automated method is suitable for high-throughput clinical laboratory settings.
    • Offers a reliable alternative for routine blood group screening.