Method for ABO Blood Group Testing Using a General-Purpose Automated Biochemical Analyzer
Related Experiment Video
Updated: Jan 23, 2026

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
Published on: March 22, 2022
Background:
We investigated a high-throughput and high-precision forward ABO blood typing screening method that utilizes a general-purpose biochemical analyzer to perform direct red blood cell sampling.
Methods:
The blood group antisera used were Ortho® BioClone® Anti-A Serum and Ortho® BioClone® Anti-B Se-rum. AFFIRMAGEN® Reagent Red Blood Cells (Ortho Clinical Diagnostics) were used for AB standard red blood cells. The general-purpose biochemical analyzer employed was the TBATM-120FR HbA1c measurement unit (Canon Medical Systems).
Results:
ABO blood group of patient samples was determined based on values relative to amount of change in the AFFIRMAGEN® response. Repeatability was CV5% or lower, and testing of 1,112 patient samples showed 100% agreement between the results obtained using the proposed method and those obtained using the tube test method.
Conclusions:
The proposed method allows ABO blood typing to be performed simply, quickly, and with a high degree of precision.
Related Concept Videos
The ABO Blood Group
Antigens in the ABO Blood Group System
Antigens are substances that can trigger an immune response, leading to the production of antibodies. In the ABO blood group system,...
Purposive Learning
Purpose of Health Records I
Here's a breakdown of how health records serve these purposes:
Purpose of Health Records II
Statistical Methods for Analyzing Epidemiological Data
Respiratory Assessment: Purpose and Indications
Objectives and Importance:
The primary goal of respiratory assessment is to evaluate patients at early risk of clinical deterioration. Since respiratory distress often precedes other signs of declining health, breathing patterns and sounds become a...

