Boar semen improvement through sperm capacitation management, with emphasis on zinc ion homeostasis

Critical to fertilization success, sperm capacitation within the female oviductal sperm reservoir endows mammalian spermatozoa with hyperactivated motility and capacity to fertilize. An elaborate cascade of signaling events during capacitation guides the redistribution of sperm plasma membrane seminolipid and cholesterol, Ca-influx and increases tyrosine phosphorylation to promote hyperactivated motility. Such events result in the remodeling of the sperm acrosome, increased fluidity and fusability of the plasma membrane, shedding of surface-adsorbed seminal plasma proteins that glue sperm heads to the oviductal epithelium and ultimately the release of hyperactivated spermatozoa from the oviductal sperm reservoir. Discovered recently, the capacitation-induced sperm zinc ion efflux and resultant zinc signatures are reflective of sperm capacitation status and fertilizing ability, inspiring the retrospection of zinc ion functions in the physiology and fertility of boar sperm and that of other species. This review also highlights the merit of the domestic boar as a biomedical model for spermatology and fertilization research. Relevant to the quest for better fertility management in the livestock industries, the benefits of zinc ion supplementation through nutrition and direct addition to extended semen are discussed in the context of artificial insemination (AI). Ideas are shared on future technologies for zinc management in AI doses and research on the sperm zinc-interacting proteome.